[BioC] Rma method for library xps

carissimo at tigem.it carissimo at tigem.it
Thu Mar 26 14:28:08 CET 2009


Dear all,

j'm using the library xps in order to analyse some .cel file from Human
Gene 1.0 st.
J have the Scheme for the chip and the Root Tree for the Cel files, when J
use the RMA method to compute expression levels, in the output file j have
only 25927 records       instead of 33297 that is the total number of
transcript units.
J used this instruction
data.rma<-
rma(data.banfi,"DataRma",filedir=datadir,tmpdir="",background="antigenomic",
       normalize=T,exonlevel="metacore+affx")

Where is the problem?

Thank you

Annamaria Carissimo




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