[BioC] tissue contamination problem in microarray

[Hao Liu]

hi! Wolfgang:

I actually talked with some very good pathologists, according to them
even microdissected sample (cancer tissue) could have around 20%
contamination from surrounding tissue.

Your answer to  my question makes me wonder if I heard is true, can
someone help to clarify this issue? Thanks

Best
Hao

On Mon, 3 May 2004, Wolfgang Huber wrote:

> 
> 
> Hao Liu wrote:
> > 
> > I would like to know if there are techniques to minimize the perturbation
> > caused by tissue contamination, for example, normal lung tissue
> > contamination on a breast metastasis. What is the best strategy to deal
> > with this problem? 
> > 
> > I think this should be dealt with at the "low" level normalization
> > process, please help.
> 
> I think it should be dealt with at the level of tissue extraction, i.e. 
> use microdissection.
> 
> Best wishes
>    Wolfgang
> 
> -- 
> -------------------------------------
> Wolfgang Huber
> Division of Molecular Genome Analysis
> German Cancer Research Center
> Heidelberg, Germany
> Phone: +49 6221 424709
> Fax:   +49 6221 42524709
> Http:  www.dkfz.de/abt0840/whuber
> -------------------------------------
> 

Best regards

Hao Liu, Ph. D