[BioC] Error While running DESeq
Simon Anders
anders at embl.de
Sat Jul 5 12:19:58 CEST 2014
Hi Nisha
First the standard advices:
1. Please always post your complete code and the seesion info. Please do
read the posting guide.
2. Please consider using DESeq2 rather the DESeq.
3. As you do not have replicates, you cannot perform a proper
statistical analysis anyway.
Now to your data:
4. Your read count table (apart from lacking replication) looks _very_
weird. Whenever a gene has more than 2 reads in one of the samples, it
always has always _exactly_ the same count in both samples! This is a
impossible to have happened by chance. Something has gone completely
wrong in creating the table.
So, it's no wonder that the dispersion fitting function gets confused.
Simon
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