[BioC] CIGAR-aware coverage
chris warth
cswarth at gmail.com
Fri Feb 28 01:37:32 CET 2014
I would like to gather coverage data for gapped alignments. I am
using readGAlignmentsFromBam() to read a GAlignments object,
converting to an IRanges object and calling IRanges::coverage() , but
that ignores CIGAR strings.
There appears to be a message to BIOC-devel on CIGAR-aware coverage
( https://www.mail-archive.com/bioc-devel@r-project.org/msg01460.html )
that suggests using the 'GenomicAlignments' package.
As far as I can tell that is only available under 2.14, the
development version of bioconductor, which would also require running
R-devel.
Is there any way to get CIGAR-aware coverage under bioconductor 2.13?
Thanks in advance,
Chris Warth
Fred Hutchinson CRC
P.S. Not to distract from the above question, but I would also like
CIGAR-aware retrieval of alignments. readGAlignmentsFromBam()
retrieves alignments whose gap completely spans the range I am
interested in. I haven't found a way to exclude alignments that
don't have any actual base alignments overlapping the range I asked
for.
====
> sessionInfo()
R version 3.0.2 (2013-09-25)
Platform: x86_64-unknown-linux-gnu (64-bit)
locale:
[1] C
attached base packages:
[1] parallel stats graphics grDevices utils datasets methods
[8] base
other attached packages:
[1] wavelets_0.3-0 vegan_2.0-10 lattice_0.20-24
[4] permute_0.8-3 rtracklayer_1.21.14 Rsamtools_1.13.52
[7] Biostrings_2.29.19 BiocInstaller_1.12.0 GenomicRanges_1.14.4
[10] XVector_0.1.4 IRanges_1.20.6 BiocGenerics_0.7.8
loaded via a namespace (and not attached):
[1] BSgenome_1.29.1 RCurl_1.95-4.1 XML_3.98-1.1 bitops_1.0-6
[5] compiler_3.0.2 grid_3.0.2 stats4_3.0.2 tools_3.0.2
[9] zlibbioc_1.7.0
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