[BioC] Reading in mutiple files using readCtData

Norm Allaire norm.allaire at biogenidec.com
Wed Feb 26 16:49:22 CET 2014


Hi Jim,

Thanks for your help. The later, I don't know how to make a character vector. 

Norm

-----Original Message-----
From: James W. MacDonald [mailto:jmacdon at uw.edu] 
Sent: Wednesday, February 26, 2014 10:46 AM
To: Norm Allaire
Cc: bioconductor at r-project.org
Subject: Re: [BioC] Reading in mutiple files using readCtData

Hi Norm,

 From ?readCtData:

Arguments
           files          character vector with the names of the files 
to be read.

So are you asking if you can pass multiple files, or are you unsure how to make a character vector?

Best,

Jim



On 2/26/2014 10:05 AM, Norm Allaire wrote:
> Hi All,
>
> I'm new to R. I just installed the HTqPCR package and I'm trying to create a qPCRset object from 8 Fluidigm files. I got some help earlier to read in one file but now I need to read in the whole set. Also, when I read in the single file the sample names were listed as 1.file name, 2.file name etc. and I would like to know how to specify the correct names that are in the file. The previous command I used was:
>> temp <- readCtData(files = "ALS_Plate1_Rep1_Results_1361960062.csv", 
>> path = "C:/Users/allairen/R/Fluidigm", n.features = 96 * 96, flag = 
>> 9, feature =5, type = 6, Ct =7, position= 1, skip=12, sep=",")
> The files I would like to read in are:
>
> ALS_Plate1_Rep1_Results_1361960062
> ALS_Plate1_Rep2_Results_1361960064
> ALS_Plate1_Rep3_Results_1361960066
> ALS_Plate1_Rep4_Results_1361960068
> ALS_Plate2_Rep1_Results_1361960063
> ALS_Plate2_Rep2_Results_1361960065
> ALS_Plate2_Rep3_Results_1361960067
> ALS_Plate2_Rep4_Results_1361960069
>
> Any help or suggestions would be greatly appreciated!
>
> Thanks so much,
>
> Norm
>
>
> Norm Allaire
> Scientist II
> Genetics and Genomics
> Biogen Idec
> 14 Cambridge Center
> Cambridge, MA 02142
> Office:  617-679-2932
> e-mail:  
> norm.allaire at biogenidec.com<mailto:norm.allaire at biogenidec.com>
>
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--
James W. MacDonald, M.S.
Biostatistician
University of Washington
Environmental and Occupational Health Sciences
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