[BioC] problem with qPCR raw data

[Heidi Dvinge]

Hello Alberto,

it's not in BioConductor, and I've never used it myself, but the  
package qpcR might be able to do what you're after.  As far as I  
remember it's on CRAN, but if not you can get it from http://www.dr- 
spiess.de/qpcR.html

Cheers
\Heidi


On 19 Jan 2010, at 11:09, Alberto Goldoni wrote:

> Dear all,
> there is a method to calculate the Ct value if i have the file.txt  
> with the fuolescence intensity like this one:
>
> Raw Data 	
> 	Experiment - PIASTRA 1 ALL+CML (Run on LCS480 1.2.9.11) 	
> 	
> 	
> 	
> 	
> SamplePos 	SampleName 	Prog# 	Seg# 	Cycle# 	Time 	Temp 	483-533
> A1 	4200_ABL 	2 	2 	1 	420616 	59.93 	6.67
> A1 	4200_ABL 	2 	2 	2 	529833 	59.93 	6.72
> A1 	4200_ABL 	2 	2 	3 	639216 	59.96 	6.86
> A1 	4200_ABL 	2 	2 	4 	748850 	59.96 	6.94
> A1 	4200_ABL 	2 	2 	5 	858416 	59.96 	7.53
> A1 	4200_ABL 	2 	2 	6 	967600 	59.96 	7.59
> A1 	4200_ABL 	2 	2 	7 	1076816 	59.96 	7.64
> A1 	4200_ABL 	2 	2 	8 	1186033 	59.93 	7.69
> A1 	4200_ABL 	2 	2 	9 	1295616 	59.96 	7.74
> A1 	4200_ABL 	2 	2 	10 	1404816 	59.96 	7.77
> A1 	4200_ABL 	2 	2 	11 	1513816 	59.96 	7.79
> A1 	4200_ABL 	2 	2 	12 	1623000 	59.96 	7.86
> A1 	4200_ABL 	2 	2 	13 	1732650 	59.96 	7.87
> A1 	4200_ABL 	2 	2 	14 	1841800 	59.96 	7.92
> A1 	4200_ABL 	2 	2 	15 	1951033 	59.96 	7.93
> A1 	4200_ABL 	2 	2 	16 	2060000 	59.96 	7.95
> A1 	4200_ABL 	2 	2 	17 	2169216 	59.96 	7.99
> A1 	4200_ABL 	2 	2 	18 	2278233 	59.96 	8
> A1 	4200_ABL 	2 	2 	19 	2387216 	59.93 	8
> A1 	4200_ABL 	2 	2 	20 	2496433 	59.96 	8.03
> A1 	4200_ABL 	2 	2 	21 	2605616 	59.93 	8.05
> A1 	4200_ABL 	2 	2 	22 	2714816 	59.96 	8.06
> A1 	4200_ABL 	2 	2 	23 	2824016 	59.96 	8.08
> A1 	4200_ABL 	2 	2 	24 	2933183 	59.96 	8.12
> A1 	4200_ABL 	2 	2 	25 	3042433 	59.96 	8.16
> A1 	4200_ABL 	2 	2 	26 	3151633 	59.93 	8.22
> A1 	4200_ABL 	2 	2 	27 	3260833 	59.96 	8.3
> A1 	4200_ABL 	2 	2 	28 	3370016 	59.93 	8.42
>
>
> -- 
> ----------------------------------------
> Alberto Goldoni, PhD
> IT Lan Manager
> Medical Genetics Unit
> S. Orsola-Malpighi Hospital
> Via Massarenti n.9, Pad 11
> 40100 Bologna, Italy
> ----------------------------------------
>
> <PIASTRA 1 ALL+CML.txt>_______________________________________________
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