[BioC] problem with qPCR raw data

[Alberto Goldoni]

Dear all,
there is a method to calculate the Ct value if i have the file.txt with 
the fuolescence intensity like this one:

Raw Data 	
	Experiment - PIASTRA 1 ALL+CML (Run on LCS480 1.2.9.11) 	
	
	
	
	
SamplePos 	SampleName 	Prog# 	Seg# 	Cycle# 	Time 	Temp 	483-533
A1 	4200_ABL 	2 	2 	1 	420616 	59.93 	6.67
A1 	4200_ABL 	2 	2 	2 	529833 	59.93 	6.72
A1 	4200_ABL 	2 	2 	3 	639216 	59.96 	6.86
A1 	4200_ABL 	2 	2 	4 	748850 	59.96 	6.94
A1 	4200_ABL 	2 	2 	5 	858416 	59.96 	7.53
A1 	4200_ABL 	2 	2 	6 	967600 	59.96 	7.59
A1 	4200_ABL 	2 	2 	7 	1076816 	59.96 	7.64
A1 	4200_ABL 	2 	2 	8 	1186033 	59.93 	7.69
A1 	4200_ABL 	2 	2 	9 	1295616 	59.96 	7.74
A1 	4200_ABL 	2 	2 	10 	1404816 	59.96 	7.77
A1 	4200_ABL 	2 	2 	11 	1513816 	59.96 	7.79
A1 	4200_ABL 	2 	2 	12 	1623000 	59.96 	7.86
A1 	4200_ABL 	2 	2 	13 	1732650 	59.96 	7.87
A1 	4200_ABL 	2 	2 	14 	1841800 	59.96 	7.92
A1 	4200_ABL 	2 	2 	15 	1951033 	59.96 	7.93
A1 	4200_ABL 	2 	2 	16 	2060000 	59.96 	7.95
A1 	4200_ABL 	2 	2 	17 	2169216 	59.96 	7.99
A1 	4200_ABL 	2 	2 	18 	2278233 	59.96 	8
A1 	4200_ABL 	2 	2 	19 	2387216 	59.93 	8
A1 	4200_ABL 	2 	2 	20 	2496433 	59.96 	8.03
A1 	4200_ABL 	2 	2 	21 	2605616 	59.93 	8.05
A1 	4200_ABL 	2 	2 	22 	2714816 	59.96 	8.06
A1 	4200_ABL 	2 	2 	23 	2824016 	59.96 	8.08
A1 	4200_ABL 	2 	2 	24 	2933183 	59.96 	8.12
A1 	4200_ABL 	2 	2 	25 	3042433 	59.96 	8.16
A1 	4200_ABL 	2 	2 	26 	3151633 	59.93 	8.22
A1 	4200_ABL 	2 	2 	27 	3260833 	59.96 	8.3
A1 	4200_ABL 	2 	2 	28 	3370016 	59.93 	8.42


-- 
----------------------------------------
Alberto Goldoni, PhD
IT Lan Manager
Medical Genetics Unit
S. Orsola-Malpighi Hospital
Via Massarenti n.9, Pad 11
40100 Bologna, Italy
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