[BioC] filetr to Agilent 4 x 44 array
Francois Pepin
francois at sus.mcgill.ca
Thu Feb 18 22:22:09 CET 2010
Hi Marco,
I have done something in the past at that particular cut-off.
I would suggest to take a look at your distribution of intensities to
make sure that 6 is a reasonable threshold. I tend to have bimodal
distributions with a sharp peak just below 6 and another later on, but
your data might be different.
Another strategy is to use the Limma F statistics, if you want to also
include genes which might be more highly expressed but also don't have
any variations.
Hope this helps,
Francois
On Feb 18, 2010, at 1:45 AM, Marco Fabbri wrote:
> HI all,
> I remeber that when I worked with affy chip , removing those genes
> that appear not to
> be expressed in either sample, value smaller than 6.
> I was wondering if I can apply a similar filter to Agilent 4 x 44 gene
> expression arrays.
>
> THank you
>
> MArco
>
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