[BioC] HTqPCR - how to read data file with multiple samples
Heidi Dvinge
heidi at ebi.ac.uk
Tue Feb 16 10:10:42 CET 2010
Hello Marco,
this feature is not available in HTqPCR 1.1.0, the release version.
If you use a newer version, available either from the BioConductor
development repository or http://www.ebi.ac.uk/bertone/software.html
they'll be a parameter n.data in readCtData().
NB: Technically this is for the R devel version, but at least on my
computer it works with R-2.10.0.
HTH
\Heidi
On 16 Feb 2010, at 08:46, Marco Fabbri wrote:
> Hi,
> I am trying ti import into HTqPCR a TLDA with two sample, I have
> seen a previous post but I ma not able to make it works, it
> recogniseas a single sample but I have a two samples.
>
> I have isntalled HTqPCR 1.1.o on a windows machine
>
>
> # Plate Pos Flag Sample Detector Task Ct
> 1 Control A1 Passed Sample01 Gene1 Endogenous Control 11.463166
> 2 Control A2 Passed Sample01 Gene2 Target 33.949196
> 3 Control A3 Passed Sample01 Gene3 Target 27.956657
> 4 Control A4 Passed Sample02 Gene1 Target 26.92745
> 5 Control A5 Passed Sample02 Gene2 Target 24.992647
> 6 Control A6 Flagged Sample02 Gene3 Target 11.280299
>
> An object of class "qPCRset"
> Size: 384 features, 1 samples
> Feature types: Endogenous Control, Target
> Feature names: CYP1A1-Hs00153120_m1 CYP1A1-Hs00153120_m1
> CYP1A1-Hs00153120_m1 ...
> Feature classes:
> Feature categories: OK, Undetermined
> Sample names: Documents and
> Settings/fabbrmc/Desktop/card1/card1 NA NA ...
>
>
> Thank you
>
> Marco
>
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