[BioC] LIMMA MZ Twin design
m.falchi at imperial.ac.uk
Fri Mar 27 14:51:23 CET 2009
I'm trying to use Limma to analyse some nimblegen methylation data on MZ
twins (imported through Ringo).
The design is:
Two colours microarray. One channel with a twin, the other channel with
the other twin
After 22 arrays we will observe the same pair (a twin for channel) but
10 years later:
I'd like to see:
How similar are the MZ twins within each array 1a Vs 1b, etc
How they change after 10 years (diverge? converge?)
How each individual changes after 10 years (1a against 1aAfter10)
The data are organized in regions of interest, therefore a variable
number of probes exist for each region.
It would be interesting comparing the methylations a whole, but also
comparing the different region of interest.
I find extremely difficult generating the design for this experiment and
I'd be extremely grateful if you could help me.
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