[BioC] differential expression methods

[Wei Shi]

Hi Mohamed:

   You have to normalize your data before you use lmFit and eBayes.

Cheers,
Wei

Mohamed lajnef wrote:
> Dear All,
>
> I have data from illumina  experiments (one colora data) (48803 rows, 120
> colones (40 colones for each  treatment) ) and I am looking for the genes
> differentially expressed by treatment
>
> could you tell me please, if i can use the package limma to apply the
> differential expression methods (lmFit,eBayes and topGene) or other method
> appropriate to my data ( for instance ANOVA)?
>
>
> Best regards
>
> ML
>
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>
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