[BioC] Error: All CEL files must be of the same type when running CRLMM
Henrik Bengtsson
hb at stat.berkeley.edu
Sun Jun 28 19:15:51 CEST 2009
The chip types are identical - it is only the CDFs that differ.
Assuming the assays didn't change too much you should be able to
analyze them together. A workaround is to create a copy of the "old"
CEL files with an updated chip type, e.g.
library("affxparser");
convertCel(filename, outFilename, newChipType="Mapping250K_Nsp");
/Henrik
On Sat, Jun 27, 2009 at 8:55 PM, Vincent
Carey<stvjc at channing.harvard.edu> wrote:
> Examining the platform metadata for GSE9222 we can see that there are
> "early access" Nsp arrays
> and standard 250k Nsp. I would think that your best bet is to use
> samples that are clearly from platform
> GPL3718.
>
> On Sat, Jun 27, 2009 at 6:32 PM, <mcoyne at boninc.com> wrote:
>> I downloaded a number of SNP 500K data from GEO to run with CRLMMM.
>> The following CEL files specified by GEO as NspI files.
>> > fulFileNames
>> [1] "/Users/pcoyne/B_Cluster_DBased/GSE9222/NspI/GSM234158.CEL"
>> [2] "/Users/pcoyne/B_Cluster_DBased/GSE9222/NspI/GSM234160.CEL"
>> [3] "/Users/pcoyne/B_Cluster_DBased/GSE9222/NspI/GSM234162.CEL"
>> > crlmm (fulFileNames,outputDir,verbose=TRUE,
>> pkgname="pd.mapping250k.nsp")
>> chips
>> Mapping250K_Nsp Mendel_Nsp
>> 2 1
>> Error in justCRLMMv3(filenames, outdir, batch_size = batch_size,
>> recalibrate = recalibrate, :
>> All the CEL files must be of the same type.
>> > sessionInfo()
>> R version 2.9.0 (2009-04-17)
>> i386-apple-darwin8.11.1
>> locale:
>> en_US.UTF-8/en_US.UTF-8/C/C/en_US.UTF-8/en_US.UTF-8
>> attached base packages:
>> [1] stats graphics grDevices utils datasets methods base
>>
>> other attached packages:
>> [1] pd.mapping250k.sty_0.4.1 pd.mapping250k.nsp_0.4.1 RSQLite_0.7-1
>>
>> [4] DBI_0.2-4 oligo_1.8.1
>> preprocessCore_1.6.0
>> [7] oligoClasses_1.6.0 Biobase_2.4.1
>> loaded via a namespace (and not attached):
>> [1] affxparser_1.16.0 affyio_1.12.0 Biostrings_2.12.7
>> IRanges_1.2.3
>> [5] splines_2.9.0
>> >
>> Question 1: What is Mendel_nsp? It looks like one of the file is
>> Mendel_nsp
>> Question 2: Is this an error that I can by pass with CRLMM and how?
>> or is it an error that I should report to GEO for fixitng?
>> Thanks
>> My Coyne
>> mcoyne at boninc.com
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>
>
>
> --
> Vincent Carey, PhD
> Biostatistics, Channing Lab
> 617 525 2265
>
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