[BioC] More iterations for median polish using Expresso
jchayes at mcb.umass.edu
jchayes at mcb.umass.edu
Mon Jun 1 22:32:37 CEST 2009
My name is James and I am having trouble normalizing some microarray data. I
have three microarray sets that I am trying to normalize using expresso. Here
is what I type and the error message which follows:
cphy.prenorm <- expresso(cphy.data, bgcorrect.method="rma",
+ normalize=FALSE,pmcorrect.method="pmonly",
+ summary.method="medianpolish")
background correction: rma
PM/MM correction : pmonly
expression values: medianpolish
background correcting...done.
11092 ids to be processed
| |
|####################|
Warning message:
In medpolish(log2(x), trace.iter = FALSE, ...) :
medpolish() did not converge in 10 iterations
Can I increase the number of iterations in expresso or do I have to do each step
one at a time? Any help you can give me would be greatly appreciated.
Best Regards,
James
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