[BioC] MAplot form arrayQualityMetrics
Ingunn Berget
ingunn.berget at umb.no
Mon Aug 31 11:10:23 CEST 2009
Dear List
I have run arrayQualityMetrics with an RG list as input to look at the quality diagnostics for a set of cDNA arrays.
There are 28 arrays, but there are only 8 MAplots in the maplot.pdf/maplot.png files.
In the other plots I get results for all other arrays (28 boxes in boxplots, 28 subplots in spatial plots and so on).
The command to run the arrayQualitMatrics function:
aqm.res <- arrayQualityMetrics(expressionset = RG,outdir = "AQM",
force=TRUE, intgroup = "Cy3",grouprep = TRUE,do.logtransform = TRUE)
I also get a lot of warnings:
Warning messages:
1: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth) :
Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'
2: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth) :
Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'
3: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth) :
Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'
4: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth) :
Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'
5: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth) :
Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'
6: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth) :
Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'
I hope that someone can give me some hints of why I don't see MAplot for all arrays, and a solution to the warnings
> sessionInfo()
R version 2.9.2 (2009-08-24)
i386-pc-mingw32
locale:
LC_COLLATE=Norwegian (Bokmål)_Norway.1252;LC_CTYPE=Norwegian (Bokmål)_Norway.1252;LC_MONETARY=Norwegian (Bokmål)_Norway.1252;LC_NUMERIC=C;LC_TIME=Norwegian (Bokmål)_Norway.1252
attached base packages:
[1] stats graphics grDevices datasets tcltk utils methods base
other attached packages:
[1] arrayQualityMetrics_2.2.2 affyPLM_1.20.0 preprocessCore_1.6.0 gcrma_2.16.0
[5] Biostrings_2.12.8 IRanges_1.2.3 affy_1.22.1 Biobase_2.4.1
[9] plyr_0.1.9 limma_2.18.2 svSocket_0.9-43 svMisc_0.9-48
[13] TinnR_1.0.3 R2HTML_1.59-1 Hmisc_3.6-1
loaded via a namespace (and not attached):
[1] affyio_1.12.0 annotate_1.22.0 AnnotationDbi_1.6.1 beadarray_1.12.0 cluster_1.12.0
[6] DBI_0.2-4 genefilter_1.24.2 grid_2.9.2 hwriter_1.1 KernSmooth_2.23-2
[11] lattice_0.17-25 latticeExtra_0.6-1 marray_1.22.0 RColorBrewer_1.0-2 RSQLite_0.7-2
[16] simpleaffy_2.20.0 splines_2.9.2 stats4_2.9.2 survival_2.35-4 tools_2.9.2
[21] vsn_3.12.0 xtable_1.5-5
>
Best regards
Ingunn
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