[BioC] combineaffybatch and Subsscript out of bound
Wolfgang Huber
huber at ebi.ac.uk
Sat Jun 21 20:01:23 CEST 2008
Dear Greg
it seems difficult to do much about your problem without being able to
reproduce it. Would it be possible for you to reproduce your problem
with just *two* CEL files (one of each type), and then making these (or
better even, the corresponding AffyBatches saved using "save"), as well
as your script, available to this list?
Also, if you are adventurous, you could use the debugger with
options(error=recover)
and explore the local variables and code in the combineAffyBatch
function where the error occurs.
Thanks and best wishes
Wolfgang.
gregory voisin wrote:
> Hi ,
> I try to use combineAffyBatch in my experiment:
>
> 2experiment using Hgu133a (10 versus 10) et hg1332plus( 3 versus 3),
> R version 2.7.0 (2008-04-22)
> x86_64-unknown-linux-gnu
> 8 gig RAM
> other attached packages:
> Â [1] hgu133plus2probe_2.2.0 hgu133plus2cdf_2.2.0Â Â hgu133plus2.db_2.2.0
> Â [4] hgu133plus2_2.2.0Â Â Â Â Â hgu133aprobe_2.2.0Â Â Â Â hgu133acdf_2.2.0
> Â [7] hgu133a_2.2.0Â Â Â Â Â Â Â Â Â hgu133a.db_2.2.0Â Â Â Â Â Â AnnotationDbi_1.2.1
> [10] RSQLite_0.6-8Â Â Â Â Â Â Â Â Â DBI_0.2-4Â Â Â Â Â Â Â Â Â Â Â Â Â matchprobes_1.12.0
> [13] RColorBrewer_1.0-2Â Â Â Â affy_1.18.1Â Â Â Â Â Â Â Â Â Â Â preprocessCore_1.2.0
> [16] affyio_1.8.0Â Â Â Â Â Â Â Â Â Â Biobase_2.0.1Â Â Â Â Â Â Â Â Â limma_2.14.2
>
> I have reproduce my code on 3 differents Unix machines
> the used classic code (is fonctionnal)Â :
>
> targets_Berthiaume = readTargets("Berthiaume_CEL/target_CUFI_NULI.txt", sep="" )
> Â abatch_Berthiaume = ReadAffy(filenames = targets_Berthiaume$FileName, celfile.path= "Berthiaume_CEL")
> targets_Zabner = readTargets("Zabner_CEL/target_CF_NCF.txt", sep="")
> abatch_Zabner = ReadAffy(filenames = targets_Zabner$FileName, celfile.path= "Zabner_CEL")
> ##################################################################
>  abatch_combin <- combineAffyBatch(list(abatch_Berthiaume, abatch_Zabner), c("h gu133aprobe", "hgu133plus2probe"), newcdf="hgu133combin", verbose=TRUE)
>
> package:hgu133aprobe   hgu133aprobe
> ackage:hgu133plus2probe       hgu133plus2probe
> 241837 unique probes in common
> Error: subscript out of bounds
> I appreciate your help to interprete this message (I Imagine that is linked to the length of data), and to solve it
> Thanks
> Â
> VOISIN Greg.
> Etudiant au Doctorat en Bioinformatique
> Université de Montreal
> Centre de recherche du CHUM.
> Institut de Recherche en Immunologie et Cancérologie
>
>
>
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