[BioC] probeset level visualization with standard deviation...
Paul Hammer
hammer_p at molgen.mpg.de
Wed Feb 20 18:12:35 CET 2008
hi members,
i am looking for a function which plots me average probeset levels with
standard deviations (e.g. tissues 1 against tissue 2). Is there any
functions which could do this. i work with affymetrix chips which have
often three times determination.
an example for a better understanding:
after using the functions read.exon() and rma() i specify my probeset_ids:
> probeset = gene.to.probeset("ensemle_gene_id")
> exon_probesets = select.probewise(probeset, filter="exonic")
now i would like to have the average values with the standard deviation
for these probeset_id log levels to plot e.g. two tissues (here heart
and breast) against later.
probeset_id heart_A.CEL heart_B.CEL heart_C.CEL
breast_A.CEL breast_B.CEL breast_C.CEL
1. 6.5432 6.7833 6.3234
4.6566 5.2121 4.7654
2. 5.8422 6.0813 6.2334
5.2526 5.1236 4.9234
3. 5.8422 6.0813 6.2334
5.2526 5.1236 4.9234
is there any simple way? till now i extract all data from a data.frame
given by splicing.index(), calculate average and sd for every
probeset_id and tissue, which is quite complicated.
thanks
paul
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