[BioC] Limma: Nimblegen array data import?

Mark Robinson mrobinson at wehi.EDU.AU
Thu Nov 29 04:58:24 CET 2007


How about just running 'limma' on the table of normalized expression  
values?

In ?lmFit, the object which gets operated on doesn't have to be an  
exprSet.

M.

On 29/11/2007, at 2:52 PM, Dave Berger wrote:

> previously, I have used limma for 2 colour array analysis.
> I now have a new data set from Nimblegen arrays in which RMA
> normalization has been completed and I wish to identify differentially
> expressed genes from the allcalls.txt file which is a table of
> expression values for all the treatments in one file
> Question:
> if I wish to do a single channel analysis in "limma", I would
> appreciate suggestions on importing this data ie. how do I convert the
> data to an "exprSet" object?
>
> thanks
> Dave Berger
>
>
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