[BioC] limma: get all sig genes from multiple contrasts
kfbargad at ehu.es
kfbargad at ehu.es
Fri Jun 9 09:03:27 CEST 2006
If I am not wrong, section 8.6 of the limma manual explains what you
want, and that is
fit2$F.p.value
hth,
David
> I realize this may be a silly question, but I have gone through all
the
> case studies in the limma users manual and I can't seem to find the
> answer to this anywhere. I have a 3x3 factorial experiment and I
> followed the case studies to make my linear model and designate 13
> contrasts of interest. My question is: is there a simple way to get
all
> the genes that show significant differences in any one of my
contrasts?
> (for use in clustering, for ex.)
> I manged to pull them out using:
>
> results <- decideTests(fit2)
> abres <- abs(results)
> sig_gene <- abres[rowSums(abres) > 0,]
>
> which then has to be linked up by
>
> test <- match(rownames(sig_gene), geneNames(eset))
> sigchange <- eset[test]
>
> This seems rather ungainly when compared to how elegantly all the
other
> functions in limma work. Is there a simpler way to do this?
>
> ivan
>
>
>
> --
>
> **************************************
> Ivan Baxter
> Post-Doc
> Purdue University
> ibaxter at purdue.edu
>
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