[BioC] limma: get all sig genes from multiple contrasts
Ivan Baxter
ibaxter at purdue.edu
Fri Jun 9 08:35:25 CEST 2006
I realize this may be a silly question, but I have gone through all the
case studies in the limma users manual and I can't seem to find the
answer to this anywhere. I have a 3x3 factorial experiment and I
followed the case studies to make my linear model and designate 13
contrasts of interest. My question is: is there a simple way to get all
the genes that show significant differences in any one of my contrasts?
(for use in clustering, for ex.)
I manged to pull them out using:
results <- decideTests(fit2)
abres <- abs(results)
sig_gene <- abres[rowSums(abres) > 0,]
which then has to be linked up by
test <- match(rownames(sig_gene), geneNames(eset))
sigchange <- eset[test]
This seems rather ungainly when compared to how elegantly all the other
functions in limma work. Is there a simpler way to do this?
ivan
--
**************************************
Ivan Baxter
Post-Doc
Purdue University
ibaxter at purdue.edu
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