[BioC] rflowcyt and prada questions

Nolwenn LeMeur nlemeur at fhcrc.org
Thu Jun 1 22:29:11 CEST 2006

Hi Andras,

Just a quick note to let you know that I've made some progress but I 
have not solved the problem yet.
The problem comes from the $DATATYPE where in FCS2.0 the data are stored 
as integer (I) and in FCS3.0 as single float precision (F).

I have also tried to read the files with prada and have the same problem.


dr. Treszl András wrote:

>Hi everyone,
>I am using the rflowcyt package to analyze data generated on a FACSAria 
>machine with the DiVa software. I collect data for 10 minutes after an 
>initial stimulation of the cells than I export the FCS 3.0 files to 
>rflowcyt. After reading in the data I noticed that the data on 
>channel "Time" do not correspond to the actual duration of the measurement. 
>Namely, when I collected data for 10 minutes (=600 sec), my time-scale in 
>rflowcyt spans to 250 "units", instead of 600. (I am calculating a lot of 
>statistics, thus the proper time-resolution would be very important.) 
>However, if I try to analyze the same data with another software (e. g. 
>FlowJo), the scale is correct, thus the data needed are within the file, 
>simply I am not able to extract it. What should I do? How can I get the 
>proper time-resolution?
>My second question: I have never been able to read in my FCS3.0 file from 
>the DiVa software via the prada package. Does prada recognize the DiVa-
>derived FCS3.0 files? If not, do you have an extension for it?
>Your help is greatly appreciated,
>Andras Treszl MD PhD
>First Department of Pediatrics, Semmelweis University, Budapest, Hungary
>Bókay u. 53
>H-1083 Budapest, Hungary
>treszl at gyer1.sote.hu
>Tel.: 36/1/334-3186
>Fax.: 36/1/313-8212
>Bioconductor mailing list
>Bioconductor at stat.math.ethz.ch
>Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor

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