[BioC] gcrma background correction
Sucheta Tripathy
sutripa at vbi.vt.edu
Wed Dec 6 07:05:52 CET 2006
Hi Jean, List,
Finally I managed to make the script
library(affy)
library(gcrma)
affinfo=compute.affinities("soybean")
myDat <-ReadAffy()
sessionInfo()
tmp1=bg.adjust.gcrma(myDat,optical.correct=T,affinity.info=affinfo)
run in my machine. The only thing I have noticed was it works with 2 chips
read simultaneously by ReadAffy(). For instance if I have only one CEL
file instead of 2 CEL files in the working directory then I end up having
the same error that I used to get before. Is there a reason for that?
Error Message:
--------------
Adjusting for optical effect.Done.
Computing affinities.Done.
Error in model.frame(formula, rownames, variables, varnames, extras,
extranames, : variable lengths differ (found for 'x') Execution halted
-----
my session info:
----------------
R version 2.4.0 (2006-10-03)
i686-pc-linux-gnu
locale:
LC_CTYPE=en_US;LC_NUMERIC=C;LC_TIME=en_US;LC_COLLATE=C;LC_MONETARY=en_US;LC_MESS
AGES=en_US;LC_PAPER=en_US;LC_NAME=C;LC_ADDRESS=C;LC_TELEPHONE=C;LC_MEASUREMENT=e
n_US;LC_IDENTIFICATION=C
attached base packages:
[1] "splines" "tools" "methods" "stats" "graphics" "grDevices"
[7] "utils" "datasets" "base"
other attached packages:
soybeanprobe soybeancdf gcrma matchprobes affy affyio
"1.14.0" "1.12.0" "2.7.0" "1.6.0" "1.12.1" "1.0.0"
Biobase
"1.12.2"
Thanks
Sucheta
--
Sucheta Tripathy, Ph.D.
Virginia Bioinformatics Institute Phase-I
Washington street.
Virginia Tech.
Blacksburg,VA 24061-0447
phone:(540)231-8138
Fax: (540) 231-2606
web page: http://staff.vbi.vt.edu/sutripa
blog : http://genomics-array.blogspot.com/
quick ref:
http://faculty.ucr.edu/~tgirke/Documents/R_BioCond/R_BioCondManual.html
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