[BioC] Quality checks / flagging
Gordon Smyth
smyth at wehi.edu.au
Thu Sep 18 12:51:20 MEST 2003
See function read.maimages() and the section QualityWeights in the limma
package. You can read in any weight functions or flags that you can
calculate from the GenePix files, i.e.,
RG <- read.maimages(files,source="genepix",wtfun=myweightfun)
where myweightfun is any function which you have defined which takes
Genepix data from one array as a matrix or data.frame and computes from it
a column of flags or quality weights. These weights then become part of the
component
RG$weights
Note that read.maimages() produces an RGList object which is essentially
equivalent to a marrayRaw object. You can transform it to an marrayRaw
object if you want.
Gordon
At 11:22 AM 18/09/2003, Tan, MinHan wrote:
>Hi,
>
>Sorry - this sounds like a rather elementary question - but is there any
>way I can read in selected columns into the marrayRaw object using
>read.genepix? (e.g. % > B532+2SD)
>
>Instead of performing quality flagging in GenePix, I was wondering how
>to achieve the same thing in R.
>
>Thank you!
>
>Regards,
>Min-Han
More information about the Bioconductor
mailing list