[BioC] Affy Question
Jason Skelton
jps at sanger.ac.uk
Wed Oct 22 17:30:34 MEST 2003
Hi
I'm not particulary familiar with any of the affy functions in
bioconductor i'm under the impression that all of the affy functions are
based on using PM & MM values for any analysis.....
BUT
The Custom affy chip we have doesn't have Mismatches
I'd like to use Bioconductor only using the PM values from the CEL file
& was wondering if this is possible at all in bioconductor...
If so can some suggest what functions need to be used
We can extract seperately
Normalised expression values
Background
No of probes
Gene Names
P-Values
but would prefer to be able to use the CEL file directly
and preferably the raw data
Cheers
Jason
--
--------------------------------
Jason Skelton
Pathogen Microarrays
Wellcome Trust Sanger Institute
Hinxton
Cambridge
CB10 1SA
Tel +44(0)1223 834244 Ext 7123
Fax +44(0)1223 494919
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