[R-sig-ME] Mixed Models in a very basic replication design

[Tim Richter-Heitmann]

Dear Group,

i am to plan a very basic factorial greenhouse experiment, and this time 
i will first ask for statistical advise before execution :).

It will encompass two treatment types with two levels each, two sampling 
dates with five replicates each, resulting in 2 x 2 x 2 x 5 = 40 samples.

I guess, this is a basic three way ANOVA (~ Treatment A * Treatment B * 
Time). However, the arrangement of the replicates in the greenhouse will 
be randomized. I have only a limited understanding of mixed models, 
obviously, but does the randomized location of the plant also requires 
the introduction of a random effect (~ Treatment A * Treatment B * Time 
+ (1|Location)?. How do i best code location in this case?

Thank you!

-- 
Tim Richter-Heitmann

University of Bremen
Microbial Ecophysiology Group (AG Friedrich)
FB02 - Biologie/Chemie
Leobener Straße (NW2 A2130)
D-28359 Bremen
Tel.: 0049(0)421 218-63062
Fax: 0049(0)421 218-63069