Thank you all for the suggestions. We do intend to get more RAM space. Meanwhile I shall have a look at the ShortRead package features. -- View this message in context: http://r.789695.n4.nabble.com/Error-cannot-allocate-vector-of-size-X-0-Mb-tp2539031p2540518.html Sent from the R help mailing list archive at Nabble.com.