[BioC] failing to filter ExpressionSet object using nsFilter
David [guest]
guest at bioconductor.org
Sat Jul 5 14:49:52 CEST 2014
Dear all,
I'm trying to use "nsFilter" to filter a post-RMA ExpressionSet (Mouse Gene 2.1), based on low filtering. This object was generated using the "rma" function. However, I get this:
> filtered <- nsFilter(myExpressionSet, var.filter=FALSE)
Error in (function (classes, fdef, mtable) :
unable to find an inherited method for function âcolumnsâ for signature â"AffyGenePDInfo"â
Any ideas how to troubleshoot this?
Thanks,
David
-- output of sessionInfo():
R version 3.0.2 (2013-09-25)
Platform: x86_64-apple-darwin10.8.0 (64-bit)
locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
attached base packages:
[1] grid parallel stats graphics grDevices utils datasets methods base
other attached packages:
[1] hgu95av2.db_2.10.1 BiocInstaller_1.12.1 mogene21stprobeset.db_2.13.0
[4] org.Mm.eg.db_2.10.1 VennDiagram_1.6.7 pathview_1.2.4
[7] org.Hs.eg.db_2.10.1 AnnotationDbi_1.24.0 KEGGgraph_1.20.0
[10] graph_1.40.1 XML_3.95-0.2 KEGGREST_1.2.2
[13] pd.mogene.2.1.st_2.12.1 RSQLite_0.11.4 DBI_0.2-7
[16] limma_3.18.13 oligo_1.26.6 Biostrings_2.30.1
[19] XVector_0.2.0 IRanges_1.20.7 oligoClasses_1.24.0
[22] genefilter_1.44.0 affy_1.40.0 Biobase_2.22.0
[25] BiocGenerics_0.8.0
loaded via a namespace (and not attached):
[1] affxparser_1.34.2 affyio_1.30.0 annotate_1.40.1 bit_1.1-12
[5] codetools_0.2-8 ff_2.2-13 foreach_1.4.2 GenomicRanges_1.14.4
[9] httr_0.3 iterators_1.0.7 png_0.1-7 preprocessCore_1.24.0
[13] RCurl_1.95-4.1 Rgraphviz_2.6.0 splines_3.0.2 stats4_3.0.2
[17] stringr_0.6.2 survival_2.37-7 tools_3.0.2 xtable_1.7-3
[21] zlibbioc_1.8.0
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