[BioC] help with flowQ vignette
Dan Tenenbaum
dtenenba at fhcrc.org
Tue May 14 20:35:32 CEST 2013
Hi Frederico,
On Tue, May 14, 2013 at 11:22 AM, Frederico Moraes Ferreira
<ferreirafm at usp.br> wrote:
> Hi List,
> I'm following the flowQ vignette (DataQualityAssessment.R) and get stucked
> in the transformation bellow.
> I've check manuals, google and ?transformList and can't figure out what's
> wrong.
> Please, could someone give me a hand?
>
>> load("qData.rda")
>> qData[[1]][[1]]
> flowFrame object 'pid02050'
> with 4500 cells and 8 observables:
> name desc range minRange maxRange
> $P1 FSC-A FSC-A 1024 0 1023
> $P2 SSC-A SSC-A 1024 0 1023
> $P3 FITC-A CD45RA 1024 1 10000
> $P4 PE-A CD45RO 1024 1 10000
> $P5 FL3-A CD8 1024 1 10000
> $P6 PE-Cy7-A CD4 1024 1 10000
> $P7 APC-A CD62L 1024 1 10000
> $P8 Time <NA> 1024 0 1023
> 82 keywords are stored in the 'description' slot
>> tData <- lapply(qData, function(x) transformList(colnames(x)[3:7], asinh)
>> %on% x)
> Error in FUN(X[[1L]], ...) : could not find function "%on%"
This command works for me. Can you send the output of the
sessionInfo() command (after loading flowQ)? That will help us figure
out the problem.
Thanks,
Dan
>> R.version
> _
> platform x86_64-pc-linux-gnu
> arch x86_64
> os linux-gnu
> system x86_64, linux-gnu
> status
> major 3
> minor 0.0
> year 2013
> month 04
> day 03
> svn rev 62481
> language R
> version.string R version 3.0.0 (2013-04-03)
> nickname Masked Marvel
>> biocLite()
> BioC_mirror: http://bioconductor.org
> Using Bioconductor version 2.12 (BiocInstaller 1.10.1), R version 3.0.0.
>
>
>
>
> Best,
>
> --
> Dr. Frederico Moraes Ferreira
> University of Sao Paulo
> Heart Institute, School of Medicine
> Laboratoy of Immunology
> Av. Dr. Enéas de Carvalho Aguiar, 44
> 05403-900 Sao Paulo - SP
> Brasil
>
> _______________________________________________
> Bioconductor mailing list
> Bioconductor at r-project.org
> https://stat.ethz.ch/mailman/listinfo/bioconductor
> Search the archives:
> http://news.gmane.org/gmane.science.biology.informatics.conductor
More information about the Bioconductor
mailing list