[BioC] RMA normalisation of test microarray data to training data

[James W. MacDonald]

Hi Daniel,

On 1/13/2012 8:47 AM, Daniel Brewer wrote:
> Hello,
>
> We have done some analysis on a set of Affy microarray data normalised by RMA and produced a predictor.  We would like to test this predictor on a training set we have.  Is it possible to RMA normalise the test dataset so that the probes have the same distribution as the training dataset without normalising all the data together?  Our concern is that if you normalise them all together then this would mean we would have to go through all the analyses again.

If you had used the frma package for the initial processing, then yes. 
There may even be some way to use frma with your extant processed data, 
but you would have to look to see.

Best,

Jim


>
> Thanks
>
> Dan
>

-- 
James W. MacDonald, M.S.
Biostatistician
Douglas Lab
University of Michigan
Department of Human Genetics
5912 Buhl
1241 E. Catherine St.
Ann Arbor MI 48109-5618
734-615-7826

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