[BioC] Openening qPCR file from ABI

Hooiveld, Guido Guido.Hooiveld at wur.nl
Thu Aug 9 15:45:44 CEST 2012


Hi Philippe,
If i recall correctly the package HTqPCR should be able to open ABI's SDS files.
See chapter 13.1, page 43 of the manual.
http://www.bioconductor.org/packages/2.10/bioc/html/HTqPCR.html

HTH,
Guido

--------------------------------------------------------- 
Guido Hooiveld, PhD 
Nutrition, Metabolism & Genomics Group 
Division of Human Nutrition 
Wageningen University 
Biotechnion, Bomenweg 2 
NL-6703 HD Wageningen 
the Netherlands 
tel: (+)31 317 485788 
fax: (+)31 317 483342 
email:      guido.hooiveld at wur.nl
internet:   http://nutrigene.4t.com 
http://scholar.google.com/citations?user=qFHaMnoAAAAJ
http://www.researcherid.com/rid/F-4912-2010


-----Original Message-----
From: bioconductor-bounces at r-project.org [mailto:bioconductor-bounces at r-project.org] On Behalf Of Philippe [guest]
Sent: Thursday, August 09, 2012 06:07
To: bioconductor at r-project.org; philipp.thomas at vol.at
Subject: [BioC] Openening qPCR file from ABI


Hi,

a colleague of mine gave me a 'sds' file, containing Real-Time PCR data from an ABI machine. I am currently trying to open it using the "ReadqPCR" package, but I am getting errors using it. So maybe someone knows another package/way which I could go to open this file? 

Best,
 Philippe

 -- output of sessionInfo(): 

R version 2.15.0 (2012-03-30)
Platform: i686-pc-linux-gnu (32-bit)

locale:
 [1] LC_CTYPE=de_DE.UTF-8       LC_NUMERIC=C              
 [3] LC_TIME=de_DE.UTF-8        LC_COLLATE=de_DE.UTF-8    
 [5] LC_MONETARY=de_DE.UTF-8    LC_MESSAGES=de_DE.UTF-8   
 [7] LC_PAPER=C                 LC_NAME=C                 
 [9] LC_ADDRESS=C               LC_TELEPHONE=C            
[11] LC_MEASUREMENT=de_DE.UTF-8 LC_IDENTIFICATION=C       

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
[1] ReadqPCR_1.2.0     affy_1.34.0        Biobase_2.16.0     BiocGenerics_0.2.0

loaded via a namespace (and not attached):
[1] affyio_1.24.0         BiocInstaller_1.4.7   preprocessCore_1.18.0
[4] zlibbioc_1.2.0 

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