[BioC] error when running paCalls
marco
hardstars26 at gmail.com
Wed Sep 21 03:49:52 CEST 2011
hello everyone.
here is my problem with code and output.
translation from Chinese to English was given when needed.
.gz of datafile were attached with this mail.
Thanks a lot!!
Lin Pei
### here is my code
library(oligo)
library(ff)
geneExp <- read.celfiles(filenames=c("GSM409113.CEL","GSM409114.CEL"))
esetps <- rma(geneExp,target="probeset")
callps <- paCalls(geneExp,method="PSDABG")
### here is the original output
> library(oligo)
载入需要的程辑包:oligoClasses
载入需要的程辑包:Biobase
Welcome to Bioconductor
Vignettes contain introductory material. To view, type
'browseVignettes()'. To cite Bioconductor, see
'citation("Biobase")' and for packages 'citation("pkgname")'.
================================================================================
Welcome to oligoClasses version 1.14.0
================================================================================
Large dataset support for 'oligo/crlmm': Disabled
- Load 'ff'
================================================================================
Parallel computing support for 'oligo/crlmm': Disabled
- Load 'ff'
- Load 'snow'
- Use options(cluster=makeCluster(...))
================================================================================
载入需要的程辑包:preprocessCore
Loading package bit1.1-7
package:bit (c) 2008/2009 Jens Oehlschlaegel (GPL-2)
creators: bit bitwhich
coercion: as.logical as.integer as.bit as.bitwhich which
operator: ! & | xor != ==
querying: print length any all min max range sum summary
bit access: length<- [ [<- [[ [[<-
for more help type ?bit
Loading package ff2.2-3
- getOption("fftempdir")=="/tmp/RtmpsQZPOJ"
- getOption("ffextension")=="ff"
- getOption("ffdrop")==TRUE
- getOption("fffinonexit")==TRUE
- getOption("ffpagesize")==65536
- getOption("ffcaching")=="mmnoflush" -- consider "ffeachflush" if
your system stalls on large writes
- getOption("ffbatchbytes")==16777216 -- consider a different value
for tuning your system
- getOption("ffmaxbytes")==536870912 -- consider a different value for
tuning your system
================================================================================
Welcome to oligo version 1.16.0
================================================================================
> library(ff)
载入需要的程辑包:tools
载入需要的程辑包:bit
载入程辑包:'bit'
The following object(s) are masked from 'package:base':
xor
Attaching package ff
载入程辑包:'ff'
The following object(s) are masked from 'package:utils':
write.csv, write.csv2
The following object(s) are masked from 'package:base':
is.factor, is.ordered
================================================================================
Large dataset support for 'oligo/crlmm': Enabled
- Probesets: 20,000
- Samples..: 100
- Path.....: /sdc1/linp/Data/GSE16558_RAW.tar_FILES
================================================================================
> geneExp <- read.celfiles(filenames=c("GSM409113.CEL","GSM409114.CEL"))
Loading required package: pd.hugene.1.0.st.v1
Loading required package: RSQLite
Loading required package: DBI
Platform design info loaded.
> esetps <- rma(geneExp,target="probeset")
Background correcting... OK
Normalizing... OK
Summarizing... OK
> callps <- paCalls(geneExp,method="PSDABG")
Computing DABG calls... 错误于log(paCalls(x, method = "DABG", verbose =
FALSE)) : (translation:error in)
数学函数中用了非数值参数 ( translation:mathematical function use non-numeric argument)
此外: 警告信息:(translation:warnings)
In `[.ff_array`(exprs(x), tmp[["fid"]], , drop = FALSE) :
opening ff /sdc1/linp/Data/GSE16558_RAW.tar_FILES/intensities-609fb008.ff
2011/9/21, Valerie Obenchain <vobencha at fhcrc.org>:
> Hello,
>
> There isn't enough information here to help answer your question.
> Please provide a small reproducible example of your error and the output
> of sessionInfo().
>
> Valerie
>
>
>
> On 09/20/2011 07:31 AM, marco wrote:
>> Hello Everyone
>> i used the function paCalls in package oligo to calculated
>> detection pvalue for human gene 1.0 ST array at probeset level but
>> fail with the error message:
>> "error in log(paCalls(x,method="DABG",verbose=FALSE))
>> mathematical function used non-numeric parameter".( message was
>> translated from Chinese)
>> my data is GSE16558 on GEO. Package ff was loaded.
>> both read.celfiles() and rma() work fine.
>> i used paCalls by paCalls(geneExp,method="PSDABG")
>> Could you please give a hand?
>> Thanks a lot!!
>>
>>
>
>
--
Lin Pei
More information about the Bioconductor
mailing list