[BioC] edgeR-DeGseq

[Gordon K Smyth]

Dear Rakhs,

See Section 9 "What to do if you don't have replicates" in the edgeR 
Users' Guide:

http://www.bioconductor.org/packages/2.9/bioc/vignettes/edgeR/inst/doc/edgeRUsersGuide.pdf

Best wishes
Gordon

> Date: Thu, 8 Sep 2011 18:28:19 +0200
> From: rakhs shit <rakhs2011 at gmail.com>
> To: bioconductor at r-project.org
> Subject: [BioC] edgeR-DeGseq
>
> Dear All ,
> I am analyzing the Rna-seq data from one sample. I don't have replicates. I
> have different cell lines (6) from one sample and I am interested in varying
> genes among different cell lines within the sample. Ideally, I would be
> interested to find differentially expressed genes in all 36 possible
> combinations. I have count data for all cell lines. So whether I should
> analyze by edgeR or DeGseq? Can I perform exact test/GLM on this sort of
> data.
> I am very new to analyze this kind of data and I would appreciate any
> guidance on this regard.
> Best
> Rakhs

______________________________________________________________________
The information in this email is confidential and intend...{{dropped:4}}