[BioC] Binomial regression of HTqPCR data

[Christopher Howerton]

Hello,

I have been entertaining the notion that high Ct values, which are generally used as an indicator of no replication due to various reasons, might actually be a response worth prediction via a binomial regression. So for example, does a predictor variable (Sex, treatment, etc.) have a significant effect on the probability of a high or "undetermined" Ct. Is this sound reasoning? If so, does anyone know of an implementation to apply a binomial regression to high-throughput data? (In my case, it would be a miR HTqPCR array).

Thanks for any discussion/suggestions,

Chris

-- 
Christopher Howerton, PhD
Postdoctoral Researcher
(215) 898-1368
University of Pennsylvania
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Philadelphia, PA 19104-6046