[BioC] annotation file and expression set genechip miRNA 2 array
Attia [guest]
guest at bioconductor.org
Fri Oct 21 14:10:12 CEST 2011
Hi Sean
Thanks for your response but I did not get how does it work after I use read.table to read the annotation file in to a data frame
For miRNA 2.0 Array annotation file from affymatrix siteFOR my miRna data
spleen.affy = ReadAffy()
sprma<-rma(spleen.affy)
pData(spleen.affy)[,1]<-c("N","N","N","P","P","P","P","P","P","N","N","N")
exprs(sprma)
here I want to apply annotation file read in data frame
to my data how to go about it
thanks
-- output of sessionInfo():
> sessionInfo()
R version 2.13.1 (2011-07-08)
Platform: x86_64-pc-mingw32/x64 (64-bit)
locale:
[1] LC_COLLATE=English_Ireland.1252 LC_CTYPE=English_Ireland.1252
[3] LC_MONETARY=English_Ireland.1252 LC_NUMERIC=C
[5] LC_TIME=English_Ireland.1252
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] mirna20cdf_2.8.0 affy_1.30.0 Biobase_2.12.2
loaded via a namespace (and not attached):
[1] affyio_1.20.0 preprocessCore_1.14.0 tools_2.13.1
>
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