[BioC] Affy U133 array CEL files - how can i see individual probe signals?

Sean Davis sdavis2 at mail.nih.gov
Wed Nov 23 13:33:17 CET 2011


On Wed, Nov 23, 2011 at 7:17 AM, Deb [guest] <guest at bioconductor.org> wrote:
>
> Hi
>
> Normally,in order to view probe signals from an Affy U133 array i import the CEL using
>
> x<-ReadAffy(*.CEL)

Hi, Deb.

The object "x" above is of class "AffyBatch".  Try taking a look at
the help for AffyBatch.

Sean


> Then have to
>
> y<-rma(x)
>
> Which only allows me to see the mean signal for all probes of the same ID, even though they represent different sequences associating with the same target gene.
>
> I want to be able to see the signal for every individual probe on the array.
>
> Does anyone know of a way of doing this?
>
> I have also converted the .CEL file to .txt using Affymetrix Power Tools but it doesn't give me Probe ID names, just x/y co-ords of the probes.
>
> Cheers.
> Deb
>
>
>
>  -- output of sessionInfo():
>
> N/A
>
> --
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>
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