[BioC] Conversion of pfm to pwm using PWM()
Ravi Karra
ravi.karra at gmail.com
Fri May 27 00:51:49 CEST 2011
Hi,
I am trying to find TF binding sites in a set of promoters using position frequency matrices (pfm's) from jaspar and transfac. I have gotten this to work before on my machine at home, but cannot seem to get this to run on campus. I am not sure why I get the following error as I thought PWM can handle integer pfm's. Any thoughts?
Thanks in advance,
Ravi
Code:
> library (Biostrings)
> pfm
[,1] [,2] [,3] [,4] [,5] [,6]
A 13 0 50 1 13 6
C 14 0 2 0 6 16
G 15 53 0 1 25 22
T 11 0 1 51 9 9
> typeof (pfm)
[1] "integer"
> pwm = pfm (PWM)
Error: could not find function "pfm"
> library (Biostrings)
> pfm
[,1] [,2] [,3] [,4] [,5] [,6]
A 13 0 50 1 13 6
C 14 0 2 0 6 16
G 15 53 0 1 25 22
T 11 0 1 51 9 9
> typeof (pfm)
[1] "integer"
> PWM (pfm)
Error in function (classes, fdef, mtable) :
unable to find an inherited method for function "PWM", for signature "matrix"
> sessionInfo ()
R version 2.12.0 (2010-10-15)
Platform: x86_64-unknown-linux-gnu (64-bit)
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
[3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
[5] LC_MONETARY=C LC_MESSAGES=en_US.UTF-8
[7] LC_PAPER=en_US.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] doMC_1.2.1 multicore_0.1-5 foreach_1.3.0 codetools_0.2-2
[5] iterators_1.0.3 Biostrings_2.18.4 IRanges_1.8.9 biomaRt_2.6.0
loaded via a namespace (and not attached):
[1] Biobase_2.10.0 RCurl_1.5-0 tools_2.12.0 XML_3.4-0
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