[BioC] arrayQualityMEtrics
Wolfgang Huber
whuber at embl.de
Wed May 25 18:50:08 CEST 2011
Dear Guillaume
Can you try again after:
library("Biobase")
What seems to happen is the following: arrayQualityMetrics wants to
convert your object 'RG' into an 'NChannelSet'. The conversion function
for doing so resides in the package vsn, and usually works. However, for
your particular object, an information message is triggered, which is
supposed to be written with the 'note' function from Biobase. It appears
that I have failed to import that into vsn. This will be fixed in
Bioc-devel in vsn >= 3.21.1.
(Perhaps I relied too much on codetools / 'R CMD check' noticing such
things - which they apparently fail to do in this case.)
Best wishes
Wolfgang
Guillaume Meurice scripsit 05/25/2011 04:27 PM:
> Dear All,
>
> I encounter the following probleme with arrayQualityMetrics (v3.8.0) :
>
>> arrayQualityMetrics(RG, outdir="aqmRaw", do.logtransform=T)
> The report will be written into directory 'aqmRaw'.
> Error in eval(expr, envir, enclos) : could not find function "note"
> Error in prepdata(expressionset, intgroup = intgroup, do.logtransform = do.logtransform) :
> Argument 'expressionset' is of class 'RGList', and its automatic conversion into 'NChannelSet' failed. Please try to convert it manually.
>
> my object RG is an RGList :
>
>> class(RG)
> [1] "RGList"
> attr(,"package")
> [1] "limma"
>>
>
> here the sessionInfo :
>
>
>> sessionInfo()
> R version 2.13.0 (2011-04-13)
> Platform: x86_64-apple-darwin9.8.0/x86_64 (64-bit)
>
> locale:
> [1] C
>
> attached base packages:
> [1] stats graphics grDevices utils datasets methods base
>
> other attached packages:
> [1] mclust_3.4.8 MASS_7.3-12 RColorBrewer_1.0-2 lattice_0.19-23 foreign_0.8-43 multicore_0.1-5
> [7] pvclust_1.2-2 arrayQualityMetrics_3.8.0 impute_1.26.0 marray_1.30.0 limma_3.8.1
>
> loaded via a namespace (and not attached):
> [1] AnnotationDbi_1.14.1 Biobase_2.12.1 Biostrings_2.20.0 Cairo_1.4-9 DBI_0.2-5 Hmisc_3.8-3 IRanges_1.10.0
> [8] KernSmooth_2.23-4 RSQLite_0.9-4 SVGAnnotation_0.9-0 XML_3.4-0 affy_1.30.0 affyPLM_1.28.5 affyio_1.20.0
> [15] annotate_1.30.0 beadarray_2.2.0 cluster_1.13.3 genefilter_1.34.0 grid_2.13.0 hwriter_1.3 latticeExtra_0.6-14
> [22] preprocessCore_1.14.0 setRNG_2009.11-1 splines_2.13.0 survival_2.36-9 tools_2.13.0 vsn_3.20.0 xtable_1.5-6
>
>
> thanks for any help
> --
> Guillaume
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--
Wolfgang Huber
EMBL
http://www.embl.de/research/units/genome_biology/huber
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