[BioC] Creating a figure showing Affy probe-set alignments along a small segment of chromosome

Vincent Carey stvjc at channing.harvard.edu
Sun May 22 04:28:51 CEST 2011


Have you examined the GenomeGraphs vignette?  There is plenty of
relevant infrastructure there.

You may also be interested in the displays provided by the ADAPT
database at Patterson Institute.

http://bioinformatics.picr.man.ac.uk/adapt/Welcome.adapt

On Sat, May 21, 2011 at 9:18 PM, Richard Student
<richard.a.student at gmail.com> wrote:
> Dear Bioconductor mailing list,
>
> If I have some CSV data in a file like this :
>
> Probe Set ID,Alignment Start,Alignment End,Strand,Gene Symbol
> 1380054_at,167415761,
> 167416381,+,Phactr3
> 1368499_at,167437521,167502083,-,Sycp2
> 1378270_at,167504376,167504858,+,Ppp1r3d
> 1373656_at,167505691,167506150,-,Ppp1r3d
> 1395392_at,167508167,167508749,-,Ppp1r3d
>
> ...
>
> 1392332_at,170985205,170985581,+,Myt1
> 1375916_at,171012460,171013368,+,Pcmtd2
> 1374454_at,171013932,171014583,+,Pcmtd2
> 1398875_at,171021421,171022475,+,Polr3k
>
> ... and I want to make a nice graph showing where each probe set aligns
> along the region between 167400000 bp and 171000000 bp, where would be a
> good place to start?  I guess I would have to use a lot of low level
> plotting functions, but I would need to create a blank graph to begin with.
> what would be cool would be a thick vertical line on the left representing
> the chromosome and vertical lines to the right showing each probeset's
> alignment to the chromosome, with each line labeled by the ProbeSetID, gene
> Symbol and strand.
>
> Any help would be greatly appreciated.
>
> Richard
>
>        [[alternative HTML version deleted]]
>
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