[BioC] FW: RE: heat map
Wolfgang Huber
whuber at embl.de
Sun May 15 10:52:01 CEST 2011
Bala
have a look at this very nice page:
http://www2.warwick.ac.uk/fac/sci/moac/students/peter_cock/r/heatmap
(It can be found by typing "heatmap.2 colsidecolors" into Google.)
Wolfgang.
Il May/12/11 8:20 AM, Reddy.Thumma at csiro.au ha scritto:
> Hi,
> I just came across the latest update on the vignette of "DESeq". I really like the new additions on visualizations particularly the visualization of expression data from genes. In my rna-seq experiment I have two conditions/treatments and three biological replication per treatment. I did the differential expression analysis and variance stabilized data using "DESeq". I want to use the VSD for generating heat map of top DE genes. I am using heatmap.2. I am able to generate a good heat map. However , I want to place an horizontal bar (below the vertical dendrogram) using 'ColSideColors' function of heat map2. I have generated the heatmap with the dendrogram of sample data (three biological replicates ie C1,C2,C3,S1,S2,S3; C=control; S=stress) on the top (vertical) of map. On the horizontal bar (below the vertical dendrogram) I want to have two colours one for each of the two treatments. I am able to add one colour to each sample using 'rainbow' and 'heat.colors' functions bu
t!
> I want to use only two colours one for each of the two conditions.
>
> Could anyone please show me how to do this.
>
> Thank you.
> Bala
>
> [[alternative HTML version deleted]]
>
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--
Wolfgang Huber
EMBL
http://www.embl.de/research/units/genome_biology/huber
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