[BioC] forge BSgenome data package
Hervé Pagès
hpages at fhcrc.org
Thu Jan 27 06:07:27 CET 2011
Steve,
On 01/26/2011 06:41 PM, Steve Shen wrote:
> Hi Herve,
>
> Sorry about that I forgot using reply all. Below is my seed file. I am
> dealing with a brand new genome that was published a couple of months
> ago. The genome consists of lots of scaffolds and contigs, no chromosome
> information available. That's why it looks weird. Yous explanation and
> suggestion does make lot of sense to me. I begin to understand some
> basic mechanism of BSgenome. This is great and thanks so much.
Good. Thanks for showing your seed file. I can confirm that you should
probably set seqnames to character(0) and mseqnames to "cflo_v3.3.fold".
However, I should point out that making a BSgenome package with a single
DNAStringSet object in it offers limited added value compared to the
solution that consists in simply loading the DNAStringSet object with
read.DNAStringSet() every time you need to work with it. The small
added value of the BSgenome wrapping here is that it's versioned and
annotated and also it makes it easier to distribute and update thru
the standard R package management infrastructure (local CRAN-style
repository / install.packages() / update.packages()).
>
> I understand now that the nmask_per_seq parameter should be set to 0
> other than 2 (which I made up previously).
Yes it will be easier if you set nmask_per_seq to 0, at least for now.
If it turns out that you really want to have masks, then you can always
add them later e.g. in the version 1.1.0 of the package.
> However, it looks to me that
> I should probably try some other strategies for this reference genome.
Good luck. Let us know how it goes.
H.
> Thanks so much for your help.
>
> Steve
>
> ## seed file start here
> Package: BSgenome.CFlo.YU.v3
> Title: Camponotus floridanus (Ants) full genome (YU version V3.3)
> Description: Camponotus floridanus (Ants) full genome as provided by YU
> (V3.3, Jan. 2011)
> Version: 1.0.0
> organism: camponotus floridanus
> species: Ant
> provider: YU
> provider_version: BGI Assembly V3.3
> release_date: Jan, 2011
> release_name: Ant Genome Reference Consortium
> source_url: NA
> organism_biocview: C_flo
> BSgenomeObjname: CFlo
> seqnames: "cflo_v3.3.fold"
> mseqnames: character(0)
> nmask_per_seq:2
> seqs_srcdir: /home/steve/Data/Genomes
> #masks_srcdir: /home/steve/Data/Masks_src
> #AGAPSfiles_name: cflo_v3.3.fa.masked
> ## seed file end here ---
>
> 2011/1/26 Hervé Pagès <hpages at fhcrc.org <mailto:hpages at fhcrc.org>>
>
> Hi Steve,
>
> Since we've started this on the list, let's keep it there.
>
> Please provide your seed file so I can get a better picture of what you
> are doing. As I already mentioned before, you need to provide 1 FASTA
> file per chromosome. But your cflo_v3.3.fold.fa file seems to contain
> 24026 sequences! So you have 2 options: (1) either you split it into 1
> file per sequence, (2) either you store it as a multiple sequence in
> your BSgenome data package.
>
> Option 1: It's easy to split a FASTA file into 1 file per FASTA
> record using the Biostrings package. You can do something like:
>
> library(Biostrings)
> x <- read.DNAStringSet("cflo_v3.3.fold.fa")
> for (i in seq_len(length(x))) {
> filepath <- paste(names(x)[i], ".fa", sep="")
> write.XStringSet(x[i], filepath)
> }
>
> The problem is that then you will need to list *all* the sequences in
> the seqnames field of your seed file. Another problem with this is that
> it's probably not the right thing to do. See below.
>
> Option 2: First some background. As you've probably noticed, the
> sequence objects stored in a BSgenome data package are divided in 2
> groups: the group of single sequences and the group of multiple
> sequences. If you've ever displayed a BSgenome object that contains
> upstream sequences, you can't have missed it. For example:
>
> > library(BSgenome.Celegans.UCSC.ce2)
> > Celegans
> Worm genome
> |
> | organism: Caenorhabditis elegans (Worm)
> | provider: UCSC
> | provider version: ce2
> | release date: Mar. 2004
> | release name: WormBase v. WS120
> |
> | single sequences (see '?seqnames'):
> | chrI chrII chrIII chrIV chrV chrX chrM
> |
> | multiple sequences (see '?mseqnames'):
> | upstream1000 upstream2000 upstream5000
> |
> | (use the '$' or '[[' operator to access a given sequence)
>
> Objects in the 1st group are DNAString (or MaskedDNAString) objects.
> Each DNAString (or MaskedDNAString) object can only contain 1 sequence.
> Objects in the 2nd group are DNAStringSet objects. Each DNAStringSet
> object can contain an (almost) arbitrary number of sequences.
> The 2 groups are respectively defined thru the seqnames and mseqnames
> fields of the seed file. I'm just paraphrasing the existing
> documentation here: all this is documented in the man page for
> BSgenome objects and in the BSgenomeForge vignette.
>
> Typically the 1st group will contain chromosome sequences, or, more
> generally (and to use Ensembl terminology), the "top-level" sequences
> of the genome. This can include the Mitochondrial DNA (often denoted
> chrM), the 2-micron plasmid, the haplotype sequences, and other exotic
> DNA material that is considered to be at the "top-level". The number
> of top-level sequences varies from one genome to the other but it
> remains generally small (< 50).
>
> Typically the 2nd group will contain 1 or more big collections of
> sequences. Each collection itself can have thousands of sequences.
> But the number of collections should preferably remain small
> (i.e. < 50). So I guess this would be the place of choice for your
> cflo_v3.3.fold object (which seems to be a collection of 24026
> scaffolds).
>
> Now for the problem with your gap file, I can't really help without
> seeing your seed file first. However, I'm a little bit puzzled that
> you have a file containing assembly gaps for your scaffolds: that
> doesn't seem to make a lot of sense to me. Anyway, you can't put
> masks on a multiple sequence object so, if you choose option 2, you
> will have to set nmask_per_seq to 0.
>
> Also, as a general rule when forging a BSgenome data package, you
> should ask yourself whether it is worth the extra effort to deal
> with the masks at all. A BSgenome data package doesn't *have* to
> contain masks.
>
> Cheers,
> H.
>
>
>
> On 01/26/2011 02:53 PM, Steve Shen wrote:
>
> Hi Herve,
>
> Thank you so much for your help. I was finally able to have a
> working
> seed file. However, there seem to be still long way to go. I
> guess that
> most likely these errors are due to genome itself and gap file.
> I just
> wonder if you could kindly give me some hints what do these error
> message mean. Is gap file required for building up data package?
> Why do
> package only use first sequence and others are truncated? Is this
> because of two many sequences? What steps can I take in order to
> make it
> work with GenomicRange packages? I really appreciate your help.
> Thanks
> so much,
>
> Steve
>
> > forgeBSgenomeDataPkg("Cflo_seed.R")
> Loading required package: Biobase
>
> Welcome to Bioconductor
>
> Vignettes contain introductory material. To view, type
> 'openVignette()'. To cite Bioconductor, see
> 'citation("Biobase")' and for packages 'citation(pkgname)'.
>
>
> Attaching package: 'Biobase'
>
> The following object(s) are masked from 'package:IRanges':
>
> updateObject
>
> Creating package in ./BSgenome.CFlo.YU.v3
> Saving 'seqlengths' object to compressed data file
> './BSgenome.CFlo.YU.v3/inst/extdata/seqlengths.rda'... DONE
> Loading FASTA file '/home/steve/Data/Genomes/cflo_v3.3.fold.fa' in
> 'cflo_v3.3.fold' object... DONE
> Saving 'cflo_v3.3.fold' object to compressed data file
> './BSgenome.CFlo.YU.v3/inst/extdata/cflo_v3.3.fold.rda'... DONE
> Error in .guessGapFileCOL2CLASS(file) :
> unable to guess the column names in "gap" file
> './cflo_v3.3.fold_gap.txt', sorry
> In addition: Warning messages:
> 1: In FUN("cflo_v3.3.fold"[[1L]], ...) :
> In file '/home/steve/Data/Genomes/cflo_v3.3.fold.fa': 24026
> sequences
> found, using first sequence only
> 2: In FUN("cflo_v3.3.fold"[[1L]], ...) :
> In file '/home/steve/Data/Genomes/cflo_v3.3.fold.fa': sequence
> description
> "scaffold9scaffold12scaffold16scaffold2scaffold4scaffold20scaffold10scaffold11scaffold24scaffold1scaffold3scaffold6scaffold15scaffold28scaffold30scaffold22scaffold18scaffold21scaffold7scaffold32scaffold36scaffold13scaffold23scaffold39scaffold19scaffold41scaffold29scaffold37scaffold33scaffold45scaffold38scaffold17scaffold5scaffold46scaffold48scaffold31scaffold25scaffold51scaffold49scaffold44scaffold47scaffold54scaffold43scaffold55scaffold60scaffold35scaffold42scaffold63scaffold53scaffold50scaffold40scaffold67scaffold61scaffold69scaffold34scaffold70scaffold27scaffold73scaffold8scaffold75scaffold71scaffold74scaffold14scaffold66scaffold65scaffold80scaffold62scaffold76scaffold79scaffold85scaffold78scaffold86scaffold88scaffold81scaffold87scaffold26scaffold92scaffold93scaffold94scaffold59scaffold52scaffold84scaffold77scaffold89scaffold100scaffold97scaffold99scaffold64scaffold103scaffold90scaffold106scaffold56scaffold98
> [... truncated]
> 3: In .forgeSeqFile(name, prefix, suffix, seqs_srcdir,
> seqs_destdir, :
> file contains 24026 sequences, using the first sequence only
> 4: In file(file, "rt") :
> cannot open file './cflo_v3.3.fold_gap.txt': No such file or
> directory
> 5: In file(file, "rt") :
> cannot open file './cflo_v3.3.fold_gap.txt': No such file or
> directory
>
>
> 2011/1/20 Hervé Pagès <hpages at fhcrc.org
> <mailto:hpages at fhcrc.org> <mailto:hpages at fhcrc.org
> <mailto:hpages at fhcrc.org>>>
>
>
> Steve,
>
>
> On 01/19/2011 04:37 PM, Steve Shen wrote:
>
> Hi Herve,
>
> Thank you very much. I tried carefully with correct path and
> file name
> by triggering auto-complete feature. But it didn't help
> either.
> It may
> be something wrong with my seed file, so I also appended the
> file below.
> Please have a look. Thanks in advance,
>
> Steve
>
> > forgeBSgenomeDataPkg("/home/steve/Data/Genomes/seed")
> Error in as.list(.readSeedFile(x, verbose = verbose)) :
> error in evaluating the argument 'x' in selecting a
> method for
> function 'as.list'
> > forgeBSgenomeDataPkg("seed")
> Error in as.list(.readSeedFile(x, verbose = verbose)) :
> error in evaluating the argument 'x' in selecting a
> method for
> function 'as.list'
>
>
> An easy way to check if your seed file is a valid DCF file is:
>
> > read.dcf("seed")
> Error in read.dcf("seed") :
> Line starting 'I'm a broken DCF fil ...' is malformed!
>
> Again, the error message you get when calling
> forgeBSgenomeDataPkg() on
> a broken DCF file should return exactly that, but,
> unfortunately, it
> doesn't with the current version of BSgenome. Updated
> versions of
> BSgenome (1.18.3 in release and 1.19.3 in devel) are
> correcting this
> and will become available tomorrow around noon, and midnight,
> respectively (Seattle time).
>
>
>
> Seed file starts:
>
> Package: BSgenome.CFlo.YU.v3
> Title: Camponotus floridanus (Ants) full genome (YU
> version V3.3)
> Description: Camponotus floridanus (Ants) full genome as
> provided by YU
> (V3.3, Jan. 2011)
>
> ^^^^^^^^^^^^^^^^^
> Is this on a line of its own in your file? This might be the
> problem.
>
> Please consult the help for the read.dcf() function
> (?read.dcf from
> your R session) for how to format a DCF file. Note that a
> 'tag:value'
> should either be placed on a single line, or, if the value spans
> several lines, then the extra lines (aka "continuation
> lines") must
> start with a whitespace. Like this:
>
>
> Description: Camponotus floridanus (Ants) full genome as
> provided
> by YU (V3.3, Jan. 2011)
>
> (I'm using 4 whitespaces here but 1 is enough. Using a <TAB>
> would
> also be OK.)
>
>
> Version: 1.0.0
> organism: camponotus floridanus
> species: Ant
> provider: YU
> provider_version: Assembly V3.3
> release_date: Jan, 2011
> release_name: Ant Genome Reference Consortium
> source_url: NA
> organism_biocview: C_flo
> BSgenomeObjname: CFlo
> seqnames: "cflo_v3.3.fold"
> mseqnames: character(0)
> nmask_per_seq:2
>
> seqs_srcdir: /home/steve/Data/Genomes/
>
>
> So you have a FASTA file named "cflo_v3.3.fold.fa" located in
> /home/steve/Data/Genomes/ that contains a *single* FASTA record
> that corresponds to the cflo_v3.3.fold sequence? Please
> double check
> this. (You can see the list of records contained in a FASTA
> file with
> the fasta.info <http://fasta.info> <http://fasta.info>()
> function from Biostrings.)
>
>
> H.
>
> # masks_srcdir: /home/steve/Data/Masks_src
> # AGAPSfiles_name: cflo_v3.3.fa.masked
>
>
> 2011/1/19 Hervé Pagès <hpages at fhcrc.org
> <mailto:hpages at fhcrc.org>
> <mailto:hpages at fhcrc.org <mailto:hpages at fhcrc.org>>
> <mailto:hpages at fhcrc.org <mailto:hpages at fhcrc.org>
> <mailto:hpages at fhcrc.org <mailto:hpages at fhcrc.org>>>>
>
> Hi Steve,
>
>
> On 01/19/2011 01:04 PM, Steve Shen wrote:
>
> Hi Herve,
>
> Thank you so much for your quick reply. Your
> vignette is
> pretty
> clear. I
> think the problem is on my side. I just lack of
> experience on
> dealing
> with this issue and maybe the file format
> doesn't fit. I
> sort of
> gave up
> using the seed file but just using low level
> commands. I
> still have
> errors. I really have no idea what is going on. Your
> help will
> be much
> appreciated.
>
> Best,
> Steve
>
> 1. with seed file, I got
> > forgeBSgenomeDataPkg("./cflo_seed.R")
> Error in as.list(.readSeedFile(x, verbose =
> verbose)) :
> error in evaluating the argument 'x' in
> selecting a
> method for
> function 'as.list'
>
>
> I agree that the error message is kind of obscure
> but the
> problem
> is probably that the specified path to your seed file is
> invalid.
> I'm about to commit a change to the BSgenomeForge
> code that will
> produce this error message instead:
>
> > forgeBSgenomeDataPkg("aaaaa")
> Error in .readSeedFile(x, verbose = verbose) :
> seed file 'aaaaa' not found
>
> An easy way to make sure the path is valid is to
> press <TAB>
> when
> you are in the middle of typing the path: this will
> trigger the
> auto-completion feature.
>
>
>
> 2. with command forgeSeqlengthsFile,
> > forgeSeqlengthsFile("cflo_v3.3.fold", prefix="",
> suffix=".fa",seqs_srcdir=".", seqs_destdir=".",
> verbose=TRUE)
> Saving 'seqlengths' object to compressed data file
> './seqlengths.rda'...
> DONE
> Warning messages:
> 1: In FUN("cflo_v3.3.fold"[[1L]], ...) :
> In file './cflo_v3.3.fold.fa': 24026
> sequences found,
> using first
> sequence only
> 2: In FUN("cflo_v3.3.fold"[[1L]], ...) :
> In file './cflo_v3.3.fold.fa': sequence
> description
> "scaffold9scaffold12scaffold16scaffold2scaffold4scaffold20scaffold10scaffold11scaffold24scaffold1scaffold3scaffold6scaffold15scaffold28scaffold30scaffold22scaffold18scaffold21scaffold7scaffold32scaffold36scaffold13scaffold23scaffold39scaffold19scaffold41scaffold29scaffold37scaffold33scaffold45scaffold38scaffold17scaffold5scaffold46scaffold48scaffold31scaffold25scaffold51scaffold49scaffold44scaffold47scaffold54scaffold43scaffold55scaffold60scaffold35scaffold42scaffold63scaffold53scaffold50scaffold40scaffold67scaffold61scaffold69scaffold34scaffold70scaffold27scaffold73scaffold8scaffold75scaffold71scaffold74scaffold14scaffold66scaffold65scaffold80scaffold62scaffold76scaffold79scaffold85scaffold78scaffold86scaffold88scaffold81scaffold87scaffold26scaffold92scaffold93scaffold94scaffold59scaffold52scaffold84scaffold77scaffold89scaffold100scaffold97scaffold99scaffold64scaffold103scaffold90scaffold106scaffold56scaffold98scaffold109scaffold68sc
> [... truncated]
>
> 3. with forgeSeqfiles,
> > forgeSeqFiles("cflo_v3.3.fold", mseqnames=NULL, prefix="",
> suffix=".fa",seqs_srcdir=".",
> seqs_destdir="./BSgenome",
> verbose=TRUE)
> Loading FASTA file './cflo_v3.3.fold.fa' in
> 'cflo_v3.3.fold'
> object... DONE
> Saving 'cflo_v3.3.fold' object to compressed
> data file
> './BSgenome/cflo_v3.3.fold.rda'... DONE
> Warning message:
> In .forgeSeqFile(name, prefix, suffix, seqs_srcdir,
> seqs_destdir, :
> file contains 24026 sequences, using the first
> sequence only
>
>
> Sorry but I can only try to help if you stick to the
> vignette and
> use a seed file. Using low-level functions like
> forgeSeqlengthsFile()
> or forgeSeqFiles() is undocumented/unsupported. The
> reason
> for this
> is that this route is *much* more complicated and
> error-prone
> than using a seed file! This is exactly why seed
> files where
> invented: to make the whole process much easier for
> you, and to
> make troubleshooting much easier for us.
>
> Cheers,
> H.
>
>
>
>
> 2011/1/19 Hervé Pagès <hpages at fhcrc.org
> <mailto:hpages at fhcrc.org>
> <mailto:hpages at fhcrc.org <mailto:hpages at fhcrc.org>>
> <mailto:hpages at fhcrc.org <mailto:hpages at fhcrc.org>
> <mailto:hpages at fhcrc.org <mailto:hpages at fhcrc.org>>>
> <mailto:hpages at fhcrc.org <mailto:hpages at fhcrc.org>
> <mailto:hpages at fhcrc.org <mailto:hpages at fhcrc.org>>
>
> <mailto:hpages at fhcrc.org <mailto:hpages at fhcrc.org>
> <mailto:hpages at fhcrc.org <mailto:hpages at fhcrc.org>>>>>
>
>
>
> Hi Steve,
>
> There are several things that look wrong
> with your
> seed file.
>
> 1. The # must be the first character in a
> line to
> make it a
> line of comment (ignored).
> For example, those 2 lines will certainly
> not be
> interpreted
> as you might expect:
>
>
> mseqnames: NA #paste("upstream",
> c("1000", "2000",
> "5000"), sep="")
>
> source_url: NA #
> http://hgdownload.cse.ucsc.edu/goldenPath/hg19/bigZips/
>
> 2. If you don't have multiple sequences,
> specify:
>
> mseqnames: character(0)
>
> 3. As explained in the BSgenomeForge
> vignette, the
> default for
> seqfiles_prefix is .fa so this won't work:
>
> seqnames: "clof_v3.fa"
>
> Unless your file is named clof_v3.fa.fa?
> If your file is named clof_v3.fa, then
> you should
> specify:
>
> seqnames: "clof_v3"
>
> 4. This doesn't look like a valid file of
> assembly gaps:
>
>
> AGAPSfiles_name: clof_v3.fa.masked
>
> Please refer to the BSgenomeForge
> vignette for
> what kind
> of masks
> and what file formats are supported.
>
> 5. Having this
>
>
> PkgExamples: Hsapiens
> seqlengths(Hsapiens)
> Hsapiens$chr1 # same as
> Hsapiens[["chr1"]]
>
> in a seed file for clof obviously doesn't
> make
> sense and your
> package won't pass 'R CMD check' because
> it will
> contain
> broken
> examples.
>
> There might be other problems with your seed
> file.
> All you
> need to do
> is read and follow carefully the instructions
> described in the
> BSgenomeForge vignette. Let me know if
> things are
> not clear
> in the
> vignette and I'll try to improve it. Thanks!
>
> H.
>
>
>
> On 01/18/2011 05:32 PM, Steve Shen wrote:
>
> Hi list,
>
> This wasn't sent out with a none
> registered ids.
> I have a
> problem with
> forging a new BSgenome data package. The
> sequence data
> file is
> clof.v3.fa
> and the mask file is clof.v3.fa.masked.
> Below
> are seed file,
> command, error
> and sessioninfo. Your help will be much
> appreciated.
>
> Thanks a lot,
> Steve
>
> Package: BSgenome.Clof.yu.v3
> Title: Clof (insects) full genome
> (version 3)
> Description: Clof (insects) full genome as
> provided by
> YU (V3.3,
> Jan. 2011)
> # and will store in Biostrings objects.
> Version: 1.0.0
> organism: clof
> species: insect
> provider: YU
> provider_version: Assembly V3.3
> release_date: Jan, 2011
> release_name: insects Genome Reference
> Consortium
> source_url: NA #
> http://hgdownload.cse.ucsc.edu/goldenPath/hg19/bigZips/
> organism_biocview: Clof
> BSgenomeObjname: Clof
> seqnames: "clof_v3.fa"
> mseqnames: NA #paste("upstream",
> c("1000", "2000",
> "5000"), sep="")
> nmask_per_seq: 2
> #SrcDataFiles1: sequences: chromFa.zip,
> upstream1000.zip,
> upstream2000.zip,
> upstream5000.zip
> #from
> http://hgdownload.cse.ucsc.edu/goldenPath/hg19/bigZips/
> #SrcDataFiles2: AGAPS masks:
> http://hgdownload.cse.ucsc.edu/goldenPath/hg19/database/gap.txt.gz
> #RM masks:
> http://hgdownload.cse.ucsc.edu/goldenPath/hg19/bigZips/chromOut.tar.gz
> #TRF masks:
> http://hgdownload.cse.ucsc.edu/goldenPath/hg19/bigZips/chromTrf.tar.gz
> PkgExamples: Hsapiens
> seqlengths(Hsapiens)
> Hsapiens$chr1 # same as
> Hsapiens[["chr1"]]
> seqs_srcdir: /home/steve/Data/Genomes
> masks_srcdir: /home/steve/Data/Genomes
> AGAPSfiles_name: clof_v3.fa.masked
>
> The command, error and sessionInfo are below
>
> forgeBSgenomeDataPkg("Clof_seed.R",
> seqs_srcdir=".",
> masks_srcdir=".",
>
> destdir=".", verbose=TRUE)
> Loading required package: Biobase
>
> Welcome to Bioconductor
>
> Vignettes contain introductory
> material. To
> view, type
> 'openVignette()'. To cite Bioconductor, see
> 'citation("Biobase")' and for packages 'citation(pkgname)'.
>
>
> Attaching package: 'Biobase'
>
> The following object(s) are masked from
> 'package:IRanges':
>
> updateObject
>
> Error in forgeBSgenomeDataPkg(y,
> seqs_srcdir =
> seqs_srcdir,
> masks_srcdir =
> masks_srcdir, :
> values for symbols NMASKPERSEQ are
> not single
> strings
> In addition: Warning message:
> In storage.mode(x$nmask_per_seq)<-
> "integer" : NAs
> introduced by
> coercion
>
> sessionInfo()
>
> R version 2.12.1 (2010-12-16)
> Platform: x86_64-pc-linux-gnu (64-bit)
>
> locale:
> [1] LC_CTYPE=en_US.UTF-8
> LC_NUMERIC=C
> [3] LC_TIME=en_US.UTF-8
> LC_COLLATE=en_US.UTF-8
> [5] LC_MONETARY=C
> LC_MESSAGES=en_US.UTF-8
> [7] LC_PAPER=en_US.UTF-8 LC_NAME=C
> [9] LC_ADDRESS=C
> LC_TELEPHONE=C
> [11] LC_MEASUREMENT=en_US.UTF-8
> LC_IDENTIFICATION=C
>
> attached base packages:
> [1] stats graphics grDevices utils
> datasets
> methods base
>
> other attached packages:
> [1] Biobase_2.6.1 BSgenome_1.18.2
> Biostrings_2.18.0
> [4] GenomicRanges_1.2.1 IRanges_1.8.8
>
> loaded via a namespace (and not attached):
> [1] tools_2.12.1
>
> [[alternative HTML version deleted]]
>
>
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>
> --
> Hervé Pagès
>
> Program in Computational Biology
> Division of Public Health Sciences
> Fred Hutchinson Cancer Research Center
> 1100 Fairview Ave. N, M2-B876
> P.O. Box 19024
> Seattle, WA 98109-1024
>
> E-mail: hpages at fhcrc.org
> <mailto:hpages at fhcrc.org> <mailto:hpages at fhcrc.org
> <mailto:hpages at fhcrc.org>>
> <mailto:hpages at fhcrc.org <mailto:hpages at fhcrc.org>
> <mailto:hpages at fhcrc.org <mailto:hpages at fhcrc.org>>>
> <mailto:hpages at fhcrc.org <mailto:hpages at fhcrc.org>
> <mailto:hpages at fhcrc.org <mailto:hpages at fhcrc.org>>
> <mailto:hpages at fhcrc.org <mailto:hpages at fhcrc.org>
> <mailto:hpages at fhcrc.org <mailto:hpages at fhcrc.org>>>>
>
>
> Phone: (206) 667-5791
> Fax: (206) 667-1319
>
>
>
>
> --
> Hervé Pagès
>
> Program in Computational Biology
> Division of Public Health Sciences
> Fred Hutchinson Cancer Research Center
> 1100 Fairview Ave. N, M2-B876
> P.O. Box 19024
> Seattle, WA 98109-1024
>
> E-mail: hpages at fhcrc.org <mailto:hpages at fhcrc.org>
> <mailto:hpages at fhcrc.org <mailto:hpages at fhcrc.org>>
> <mailto:hpages at fhcrc.org <mailto:hpages at fhcrc.org>
> <mailto:hpages at fhcrc.org <mailto:hpages at fhcrc.org>>>
> Phone: (206) 667-5791
> Fax: (206) 667-1319
>
>
>
>
> --
> Hervé Pagès
>
> Program in Computational Biology
> Division of Public Health Sciences
> Fred Hutchinson Cancer Research Center
> 1100 Fairview Ave. N, M2-B876
> P.O. Box 19024
> Seattle, WA 98109-1024
>
> E-mail: hpages at fhcrc.org <mailto:hpages at fhcrc.org>
> <mailto:hpages at fhcrc.org <mailto:hpages at fhcrc.org>>
> Phone: (206) 667-5791
> Fax: (206) 667-1319
>
>
>
>
> --
> Hervé Pagès
>
> Program in Computational Biology
> Division of Public Health Sciences
> Fred Hutchinson Cancer Research Center
> 1100 Fairview Ave. N, M2-B876
> P.O. Box 19024
> Seattle, WA 98109-1024
>
> E-mail: hpages at fhcrc.org <mailto:hpages at fhcrc.org>
> Phone: (206) 667-5791
> Fax: (206) 667-1319
>
>
--
Hervé Pagès
Program in Computational Biology
Division of Public Health Sciences
Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N, M2-B876
P.O. Box 19024
Seattle, WA 98109-1024
E-mail: hpages at fhcrc.org
Phone: (206) 667-5791
Fax: (206) 667-1319
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