[BioC] HTqPCR questions

[Heidi Dvinge]

<snip>

>> I know that some people generally don't trust commercial software much,
>> and prefer to get all the raw data, i.e. all the individual fluorescence
>> measurements, and then fit some sort of sigmoid curve manually. However,
>> I
>> ahve yet to see any conclusive evidence that this is really necessary,
>> and
>> especially worth the extra effort. Perhaps other disagree with me here?
>
> I spent some time playing with the raw data.  In part, I wanted to see
> what happened if you fit actual logistic models that account for the
> fact that some probe-primer pairs are probe limited and other are
> primer-limited. I decided that the basic model-fitting was good enough,
> in that accounting for these extra complications didn't seem to have any
> real payoff in the kinds of inferences one wanted to draw from the
> data.  None of this was published (since it's rather hard to publish
> something that says "this more detailed model with more parameters has
> no advantages over the current model for this kind of data"), and I'm
> not sure if I can even find the actual computations any more....
>
Just for the records, if anyone do indeed want to play with the raw qPCR
data, there's also this option (the first ones to snatch up the name qpcR
for a package ;)

http://bioinformatics.oxfordjournals.org/content/24/13/1549.short

\Heidi

>> HTH
>> \Heidi
>>
>>> Kind regards,
>>>
>>> Alessandro G.
>