[BioC] help with DESeq
Simon Anders
anders at embl.de
Mon Dec 12 21:11:18 CET 2011
Dear Nirmala
> We have 4 cases and 5 controls. We would like to add RIN number as one of the covariates to the data analysis. Here is my code which gives errors:
>
>> countsTable=read.table("4Cases_5Controls_counts.txt",header=TRUE)
>> cdsFull<- newCountDataSet( countsTable, nisc1Design )
>> cdsFull<- estimateSizeFactors( cdsFull )
>> cdsFull<- estimateDispersions(cdsFull,"pooled")
>> Error in rowSums(sapply(tapply((1:ncol(counts))[replicated_sample], factor(conditions[replicated_sample]), :
>> 'x' must be an array of at least two dimensions
At the very least, tell us what is the variables you use to construct
the CountDataSet, i.e., post the content of 'nisc1Design' and the head
of 'countsTable'.
Simon
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