[BioC] ddCt isn't working with exported ABI7500-RT-PACR file

[Alnaji, Fadi]

Deam Jim, thanks a lot. 

I'll try this out and let you know. 

All the best - Fadi 

-----Original Message-----
From: jimrperkins at gmail.com [mailto:jimrperkins at gmail.com] On Behalf Of James Perkins
Sent: 01 December 2011 11:42
To: Alnaji, Fadi
Cc: bioconductor at r-project.org
Subject: Re: [BioC] ddCt isn't working with exported ABI7500-RT-PACR file

Hey,

You could try ReadqPCR or HTqPCR, both enable you to read in SDS files
I believe (ReadqPCR has a read.qPCR function, and there is an
explanation of how to do so in HTqPCR). If you were to have problems
using your version of SDS file with ReadqPCR, I would be very grateful
if you could email me your file (annonymsed if you wish of course) so
I can alter ReadqPCR appropriately.

Cheers,

Jim

On 1 December 2011 10:51, Alnaji, Fadi <F.Alnaji at warwick.ac.uk> wrote:
>
> Hello
>
> I am trying to use the ddCt package which was developed by Jitao David
> Zhang, Rudolf Biczok and Markus Ruschhaupt. My problem is that it seems
> the package is not able to read the exported file, the one contains the
> individual ct Values, from ABI 7500 RT-PCR instrument. When I execute
> the package, it says your file is not .SDM. That's true, as my file is
> SDS, but this is the only possibility I have got in the machine. However
> I tried to play a bit with the text format in excel to make SDS as
> similar as the SDM in the package-example file. It worked indeed, but it
> is not a practical way I believe.
>
> Is there any other option to make this package able to read my SDS
> files, or to convert this file to SDM.
>
> Many thanks.
>
>
>
>
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