[BioC] rtracklayer::liftOver ordering
Andrew Jaffe
ajaffe at jhsph.edu
Wed Aug 24 17:28:03 CEST 2011
I'm having a problem maintaining the ordering of my GRanges object
when I lift it over using rtracklayer::liftOver. For example:
> g # my regions
GRanges with 5 ranges and 0 elementMetadata values
seqnames ranges strand |
<Rle> <IRanges> <Rle> |
[1] chr19 [ 13130686, 13133039] * |
[2] chr4 [160026138, 160028079] * |
[3] chr12 [ 65671230, 65672140] * |
[4] chr8 [ 19615409, 19616461] * |
[5] chr14 [ 99706752, 99708661] * |
> chain = import.chain("hg19ToHg18.over.chain") # from UCSC
> lifted = liftOver(g, chain) # suppressed unmatched chrs
> lifted
GRanges with 5 ranges and 0 elementMetadata values
seqnames ranges strand |
<Rle> <IRanges> <Rle> |
[1] chr4 [160245588, 160247529] * |
[2] chr8 [ 19659689, 19660741] * |
[3] chr12 [ 63957497, 63958407] * |
[4] chr14 [ 98776505, 98778414] * |
[5] chr19 [ 12991686, 12994039] * |
This is just a toy example with 5 regions all on different
chromosomes, but with real data where there are multiple regions per
chromosome, I am unable to determine the resulting matched lifted data
for a particular region. Is there any way to preserve the ordering of
my original list in the liftOver output? Presorting by chromosome and
position might work 99% of time, but the ordering of some regions
might shift during the liftOver, and I would not be able to tell if
this occurred.
Thanks a lot,
Andrew Jaffe
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