[BioC] Loading Niblegen Files to Oligo
Colleen Doherty
cdoherty at ucsd.edu
Sat Oct 23 00:56:23 CEST 2010
Hello,
We are trying to load Nimblegen Files to the Oligo Package. We do
not have .xys files so tried to make them from the .pair files
following this link:
http://permalink.gmane.org/gmane.science.biology.informatics.conductor/29947
However, when trying to read them into Oligo we get this error
> dat <- read.xysfiles(xys_files)
Platform design info loaded.
Checking designs for each XYS file... Done.
Allocating memory... Error in smartReadXYS(filenames, sampleNames) :
negative extents to matrix
I guess we do not have the .xys files made correctly, but I'm not sure
what we have incorrect, since I can't find exactly what it should look
like.
This is what we have:
# software=NimbleScan version=2.4.27 designname=090717_Athal_TAIR9_exp
X Y EOL Count
23 3 731.67 NA
135 3 317.33 NA
137 3 742.44 NA
139 3 264.89 NA
141 3 2006.22 NA
143 3 898.11 NA
Does anyone see anything obvious we have wrong?
Session info is below
Any advice would be appreciated.
Thanks very much!
Colleen
> sessionInfo()
R version 2.12.0 (2010-10-15)
Platform: i386-apple-darwin9.8.0/i386 (32-bit)
locale:
[1] en_US.UTF-8/en_US.UTF-8/C/C/en_US.UTF-8/en_US.UTF-8
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] pd.090717.athal.tair9.exp_0.0.1 RSQLite_0.9-2
[3] DBI_0.2-5 oligo_1.14.0
[5] oligoClasses_1.12.0 Biobase_2.10.0
loaded via a namespace (and not attached):
[1] affxparser_1.22.0 affyio_1.18.0 Biostrings_2.18.0
[4] IRanges_1.8.0 preprocessCore_1.12.0 splines_2.12.0
>
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