[BioC] Error Message upon filtering in AgiMicroRNA of RMA normalized object.

[Richard Friedman]

Dear Pedro and list

	I encountered an error message upon filtering an RMA normalized object.

 > ddPROC=filterMicroRna(agiMicro.rma,
+  agiMicroraw,
+ control=TRUE,
+ IsGeneDetected=FALSE,
+ wellaboveNEG=FALSE,
+   limIsGeneDetected=50,
+ limNEG=25,
+  makePLOT=TRUE,
+ targets,
+  verbose=TRUE)
FILTERING PROBES BY FLAGS


FILTERING BY ControlType
Error in data.frame(as.character(PROBE_ID), as.character(GENE_ID),  
as.character(probe.chr),  :
   arguments imply differing number of rows: 644, 0

The relevant portions of my script up until that point are:
###################################################
library("AgiMicroRna")
targets<-readTargets("Targets.txt",verbose=TRUE)
agiMicroraw<-readMicroRnaAFE(targets,verbose=TRUE)

agiMicro.rma=rmaMicroRna(agiMicroraw,normalize=TRUE,background=FALSE)

ddPROC=filterMicroRna(agiMicro.rma,
                     agiMicroraw,
                     control=TRUE,
                     IsGeneDetected=FALSE,
                     wellaboveNEG=FALSE,
                     limIsGeneDetected=50,
                     limNEG=25,
                     makePLOT=TRUE,
                     targets,
		    verbose=TRUE)
###################################################

Please advise.

Thanks and best wishes,
Rich
------------------------------------------------------------
Richard A. Friedman, PhD
Associate Research Scientist,
Biomedical Informatics Shared Resource
Herbert Irving Comprehensive Cancer Center (HICCC)
Lecturer,
Department of Biomedical Informatics (DBMI)
Educational Coordinator,
Center for Computational Biology and Bioinformatics (C2B2)/
National Center for Multiscale Analysis of Genomic Networks (MAGNet)
Room 824
Irving Cancer Research Center
Columbia University
1130 St. Nicholas Ave
New York, NY 10032
(212)851-4765 (voice)
friedman at cancercenter.columbia.edu
http://cancercenter.columbia.edu/~friedman/

In Memoriam,
George Scithers