[BioC] Questions about calculate gene signal

Wolfgang Huber whuber at embl.de
Thu Jun 17 10:55:51 CEST 2010


On Jun/17/10 6:22 AM, Yang Lv wrote:
> Dear List,
>
> I'm new to microarray analysis and need to calculate gene signal from
> samples. The tab-delimited file has been prepared and the signal for each
> probe is calculated. My question is: 1) How to filter the probes those are
> disqualified? 2) How to calculate the gene signal from probe signal? The
> reflection from probes to genes are clear but I have no idea if I need to
> simply sum up all the probes of a given gene or something else.
>
> Thanks a lot and best wishes.
>

Dear Yang

have a look at the exercise book
http://www.bioconductor.org/pub/biocases/
that discusses your questions in extenso.

  Best wishes
        Wolfgang



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