[BioC] Question on makeVennDiagram function in ChIPpeakAnno
Zhu, Julie
Julie.Zhu at umassmed.edu
Sun Jun 13 16:31:29 CEST 2010
Hi Noah,
Totaltest specifies the total number of tests performed to obtain the list
of peaks. It should be larger than the largest number of peaks in A and B.
100 is too small. Thanks!
Best regards,
Julie
On 6/12/10 9:04 PM, "Noah Dowell" <noahd at ucla.edu> wrote:
> Hello All,
>
> I ran the following function and received the following output but it is
> unclear to me why I have a negative number for vennCounts (and no p-value).
> Any ideas? My output for the example in the vignette is correct but running
> the function with my data I am getting the following:
>
>> makeVennDiagram(RangedDataList(smallAnnoTFA, smallAnnoTFB),
>> NameOfPeaks=c("TFA", "TFB"), maxgap = 0, totalTest= 100, cex = 1, counts.col
>> = "purple")
> $p.value
> [1] NaN
>
> $vennCounts
> TFA TFB Counts
> [1,] 0 0 -2667
> [2,] 0 1 755
> [3,] 1 0 1334
> [4,] 1 1 678
> attr(,"class")
> [1] "VennCounts"
>
> There were 17 warnings (use warnings() to see them)
>
>> warnings()
> Warning messages:
> 1: In .local(query, subject, maxgap, minoverlap, type, select, ... :
> argument 'multiple' is deprecated; use 'select'.
> 2: In .local(query, subject, maxgap, minoverlap, type, select, ... :
> argument 'multiple' is deprecated; use 'select'.
> 3: In .local(query, subject, maxgap, minoverlap, type, select, ... :
> argument 'multiple' is deprecated; use 'select'.
>
>
>
> Thank you for any help.
>
> Noah
>
>
>
>
>
>
>
>> sessionInfo()
> R version 2.11.0 (2010-04-22)
> i386-apple-darwin9.8.0
>
> locale:
> [1] en_US.UTF-8/en_US.UTF-8/C/C/en_US.UTF-8/en_US.UTF-8
>
> attached base packages:
> [1] grid stats graphics grDevices utils datasets methods base
>
> other attached packages:
> [1] org.Sc.sgd.db_2.4.1 rtracklayer_1.8.1
> [3] RCurl_1.3-1 bitops_1.0-4.1
> [5] Starr_1.4.0 affxparser_1.20.0
> [7] affy_1.26.0 Ringo_1.12.0
> [9] Matrix_0.999375-38 lattice_0.18-5
> [11] RColorBrewer_1.0-2 ChIPpeakAnno_1.4.0
> [13] limma_3.4.0 org.Hs.eg.db_2.4.1
> [15] GO.db_2.4.1 RSQLite_0.8-4
> [17] DBI_0.2-5 AnnotationDbi_1.10.0
> [19] BSgenome.Ecoli.NCBI.20080805_1.3.16 BSgenome_1.16.0
> [21] GenomicRanges_1.0.1 Biostrings_2.16.0
> [23] IRanges_1.6.0 multtest_2.4.0
> [25] Biobase_2.8.0 biomaRt_2.4.0
>
> loaded via a namespace (and not attached):
> [1] affyio_1.16.0 annotate_1.26.0 genefilter_1.30.0
> MASS_7.3-5
> [5] preprocessCore_1.10.0 pspline_1.0-14 splines_2.11.0
> survival_2.35-8
> [9] tools_2.11.0 XML_2.8-1 xtable_1.5-6
>>
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