[BioC] Limma -time course - no replicates

[Lana Schaffer]

Naomi,
Can you please suggest a routine or software for us to use?
Lana

________________________________________
From: Naomi Altman [naomi at stat.psu.edu]
Sent: Friday, June 04, 2010 6:49 PM
To: Lana Schaffer; bioconductor at stat.math.ethz.ch
Subject: Re: [BioC] Limma -time course - no replicates

Only if you consider this to be a regression on a function of time,
instead of an ANOVA.

--naomi

At 07:12 PM 6/4/2010, Lana Schaffer wrote:
>Hi,
>We are trying to use Limma code for a Time Course Experiment without
>Replicates for 4 times.  From looking at the manual, looks like don't
>Need replicates.  But the ebayes routine complains about no residual
>Degrees of freedom.
>Can we use Limma for Time Course without replicates?
>Lana
>
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Naomi S. Altman                                814-865-3791 (voice)
Associate Professor
Dept. of Statistics                              814-863-7114 (fax)
Penn State University                         814-865-1348 (Statistics)
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