[BioC] running out of memory

[Javier Pérez Florido]

Dear Steve,
Thanks for your help, but, I'm trying to preprocess using RMA, GCRMA, 
VSN and dChip methods and I don't see any function in aroma.affymetrix 
that performs the whole preprocessing...



Steve Lianoglou escribió:
> Hi,
>
> 2010/1/20 Javier Pérez Florido <jpflorido at gmail.com>:
>   
>> Dear list,
>> I'm trying to normalize using threestep several CEL files (around 100
>> CEL files). I got the following error:
>>
>> Error: cannot allocate vector of size 6.5 Mb
>>
>> I've been reading the suggestions about this error in the mailing list,
>> but I couldn't fix it. My system (Win XP Prof, 32 bits) has 3 GB RAM and
>> I did the following:
>>
>>    * I used the command line --max-mem-size=3071M in the R shortcut
>>    * I changed the boot.ini file to allow up to 3GB
>>          o [boot loader]
>>            timeout=30
>>            default=multi(0)disk(0)rdisk(0)partition(1)\WINDOWS
>>            [operating systems]
>>            multi(0)disk(0)rdisk(0)partition(1)\WINDOWS="Microsoft
>>            Windows XP Professional" /3GB /noexecute=optin /fastdetect
>>    * I try to remove not used variables using the rm() command and the
>>      garbage collector gc() within the source code.
>>
>> But, even with those changes, I'm still running out of memory. It is
>> strange, because, having a look at the tasks administrator, R doesn't
>> use more than 1600 MB and the whole system never goes further than 2GB
>>     
>
> I can't really comment on tweaking memory settings on windows systems,
> but if all you're doing is trying to normalize a boat-load of affy
> arrays together, I understand that the aroma.affymetrix package can do
> so while keeping memory requirements down. Perhaps you might consider
> looking into it until someone can give you better advice:
>
> http://groups.google.com/group/aroma-affymetrix/web/overview
>
> HTH,
> -steve
>
>