[BioC] background correction within beadarray
mark.dunning at gmail.com
Tue Jan 12 17:31:01 CET 2010
There are two types of background correction used for Illumina data.
The first is an image processing step and occurs at the bead-level
(e.g. in the files such as where each bead is adjusted to adjust for
local aberrations on the surface of the array. This is the
backgroundCorrect function that you find in beadarray.
The second occurs after summarisation, for example by the BeadStudio
software, where each bead-type is adjusted for non-specific
hybridisation using the values of negative controls on the same array.
However, we find this type of correction introduces missing data and
high variability so we don't usually recommend it. Background
correction of summarized data isn't currently supported by beadarray.
On Tue, Jan 5, 2010 at 10:43 AM, <gblackshields at gmail.com> wrote:
> I've recently started getting into using the beadarray package for reading
> Illumina microarray files, and am unfortunately asking questions already. I
> see that there is a background correction function for beadlevel data, but
> I can't find one yet for beadsummary data. Does beadsummary perform
> background correction automatically on the data, or should it be corrected
> within BeadStudio before exporting the files in the first place?
> thanks in advance,
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